Abstract
Thirty-one strains ofVibrio anguillarum serogroup O1 isolated in Italy and Greece from dead fish were subjected to rRNA gene restriction pattern analysis. WithHindIII as the restriction enzyme, two different ribotypes were detected, of which one profile was dominant. One profile was found in all strains except one isolated from sea bass, sea bream, and mullet, while the second profile was found in all three strains isolated from rainbow trout and in one strain from a sea bass. WithEcoRI only one profile was found.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Andersen JK, Saunders NA (1990) Epidemiological typing ofYersinia enterocolitica by analysis of restriction length polymorphisms with a cloned ribosomal RNA gene. J Med Microbiol 32:179–187
Bolinches J, Lemos ML, Fouz B, Cambra M, Larsen JL, Toranzo AE (1990) Serological relationship amongVibrio anguillarum strains. J Aquat Anim Hlt 2:21–29
Grimont F, Grimont PAD (1986) Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur/Microbiol 137B:165–175
Irino K, Grimont F, Casin I, Grimont PAD, The Brazilian Purpuric Fever Study Group (1988) rRNA gene restriction patterns ofHaemophilus influenza biogroup aegyptius stains associated with Brazilian purpuric fever. J Clin Microbiol 26:1535–1538
Larsen JL (1990)Vibrio anguillarum: characterization, ecology, and pathogenicity. Dissertation, DSR Press, Copenhagen, Denmark
Larsen JL, Rasmussen HB, Dalsgaard I (1988) Study ofVibrio anguillarum from different sources with emphasis on ecological properties. Appl Environ Microbiol 54:2264–2267
Moyer NP, Martinetti G, Lüthy-Hottenstein J, Altwegg M (1992) Value of rRNA gene restriction patterns ofAeromonas spp. for epidemiological investigations. Curr Microbiol 24:15–21
Olsen JE, Larsen JL (1990) Restriction fragment length polymorphism of theVibrio anguillarum serovar O1 virulence plasmid. Appl Environ Microbiol 56:3130–3131
Owen RJ (1989) Chromosomal DNA fingerprinting—a new method of species and strain identification applicable to microbial pathogens. J Med Microbiol 30:89–99
Pedersen K, Larsen JL (1993) rRNA gene restriction pattern analysis ofVibrio anguillarum serogroup O1. Dis Aquat Org, in press.
Rehnstam A-S, Norqvist A, Wolf-Watz H, Hagström Å (1989) Identification ofVibrio anguillarum in fish by using partial 16S rRNA sequences and a specific 16S rRNA oligonucleotide probe. Appl Environ Microbiol 55:1907–1910
Saunders NA, Harrison TG, Kachawalla N, Tailor AG (1988) Identification of species of the genusLegionella using cloned rRNA gene fromLegionella pneumophila. J Gen Microbiol 134:2363–2374
Schiewe MH, Crosa JH (1981) Molecular characterization ofVibrio anguillarum biotype 2. Can J Microbiol 27:1011–1018
Sørensen UBS, Larsen JL (1986) Serotyping ofVibrio anguillarum. Appl Environ Microbiol 51:593–597
Toranzo AE, Barja JL (1990) A review of the taxonomy and seroepizootiology ofVibrio anguillarum, with special reference to aquaculture in the northwest of Spain. Dis Aquat Org 9:73–82
Tsai H-J, Yeh M-S, Song Y-L (1990) Characterization of Vibrio species by using DNA fingerprinting techniques. Fish Pathol 25:201–206
Wards BJ, Patel HH, Anderson CD, de Lisle GW (1991) Characterization by restriction endonuclease analysis and plasmid profiling ofVibrio ordalii strains from salmon (Oncorhynchus tshawytscha andOncorhynchus nerka) with vibriosis in New Zealand. NZ J Marine Freshwater Res 25:345–350.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pedersen, K., Ceschia, G. & Larsen, J.L. Ribotypes ofVibrio anguillarum O1 from Italy and Greece. Current Microbiology 28, 97–99 (1994). https://doi.org/10.1007/BF01569054
Issue Date:
DOI: https://doi.org/10.1007/BF01569054