Abstract
A human genomic DNA segment (λhal) which hybridizes with rat pancreatic amylase cDNA was used to regionally assign amylase genes in human-mouse somatic cell hybrids. The assignment of amylase genes to chromosome 1 was confirmed and regionally mapped to the short arm region p22.1→p21 using a cell hybrid retaining a translocation involving chromosome 1 [46,XX,t(1;2)(p21;q37)]. Restriction length polymorphisms at the amylase loci are reported for gene linkage studies.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature cited
Karn, R.C., and Malacinski, G.M. (1978).Adv. Comp. Physiol. Biochem. 7:1–103.
Sanders, T.G., and Rutter, W.J. (1972).Biochemistry 11:110–136.
Schibler, U., Tosi, M., Pittet, A.C., Fabiani, L., and Wellaur, P.K. (1980).J. Mol. Biol. 142:93–116.
Merritt, A.D., and Karn, R.C. (1977). InAdvances in Human Genetics, Vol. 8, (eds.) Harris, H., and Hirschhorn, K. (Plenum Press, New York), pp. 135–234.
Kaplan, R.D., Chapman, V., and Ruddle, F.H. (1973).Heredity 64:155.
Young, R.A., Hagenbuchle, O., and Schibler, U. (1981).Cell 23:451–458.
Harding, J.D., and Rutter, W.J. (1978).J. Biol. Chem. 253:8736–8740.
Cook, P.J.L., and Hamerton, J.L. (1982).Cytogenet. Cell Genet. 32:111–120.
MacDonald, R.J., Crerar, M.M., Swain, W.F., Pictet, R.L., Thomas, G., and Rutter, W.J. (1980).Nature 287:117–122.
Shows, T.B. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:348–352.
Shows, T.B., and Brown, J.A. (1975).Proc. Natl. Acad. Sci. U.S.A. 72:2125–2129.
Davidson, R., and Gerald, P.S. (1976).Somat. Cell Genet. 2:165–176.
Lalley, P.A., Brown, J.A., Eddy, R.L., Haley, L.L., Byers, M.G., Goggin, A., and Shows, T.B. (1977).Biochem. Genet. 15:367–382.
Shows, T.B., and Brown, J.A. (1975).Cytogenet. Cell Genet. 14:251–255.
Owerbach, D., Bell, G.I., Rutter, W.J., Brown, J.A., and Shows, T.B. (1981).Diabetes 30:267–270.
Shows, T.B., Brown, J.A., Haley, L.L., Byers, M.G., Eddy, R.L., Cooper, E.S., and Goggin, A.P. (1978).Cytogenet. Cell Genet. 21:99–104.
Lalley, P.A., Brown, J.A., and Shows, T.B. (1976).Cytogenet. Cell Genet. 16:188–191.
Naylor, S.L., Elliott, R.W., Brown, J.A., and Shows, T.B. (1982).Am. J. Hum. Genet. 34:235–244.
Shows, T.B. (1977). InIsozymes: Current Topics in Biological and Medical Research, Vol. 2, (eds.) Rattazzi, M.C., Scandalios, J.G., and Whitt, G.S. (A.R. Liss, New York), pp. 107–158.
Naylor, S.L., Sakaguchi, A.Y., Shows, T.B., Law, M.L., Goeddel, D.V., and Gray, P.W. (1983).J. Exp. Med. 157:1020–1027.
Lawn, R.M., Fritsch, E.F., Parker, R.C., Blake, G., and Maniatis, T. (1978).Cell 15:1157–1174.
Southern, E.M. (1975).J. Mol. Biol. 98:503–517.
Denhardt, D.T. (1966).Biochem. Biophys. Res. Commun. 23:641–646.
Shows, T.B., Sakaguchi, A.Y., and Naylor, S.L. (1982). InAdvances in Human Genetics, Vol. 12, (eds.) Harris, H., and Hirschhorn, K. (Plenum Press, New York).
Human Gene Mapping Workshop VI (1982).Cytogenet. Cell Genet. 32:111–245.
Zabel, B., Naylor, S.L., Sakaguchi, A.Y., Bell, G.I., and Shows, T.B. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:6932–6936.
Shows, T.B., and Brown, J.A. (1975).Cytogenet. Cell Genet. 14:251–255.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tricoli, J.V., Shows, T.B. Regional assignment of human amylase (AMY) to p22 → p21 of chromosome 1. Somat Cell Mol Genet 10, 205–210 (1984). https://doi.org/10.1007/BF01534909
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01534909