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Localization of proteasomes in plant cells

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Summary

Proteasomes, also known as multicatalytic proteinase complexes, were localized in suspension cells of potato (Solanum tuberosum) by direct immunofluorescence using polyclonal antibodies labelled with fluorescein isothiocyanate. The method used allows an estimate of relative amounts of proteasomal antigens in different cell components. Proteasomes are present in the nuclei and the cytoplasm. The nucleoplasm contains small areas of weak fluorescence. The peripheral cytoplasm and possibly elements of the cytoskeleton show higher fluorescence than other parts of the cytoplasm. This indicates a localization of proteasomes similar to that known from animal cells.

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Abbreviations

DMSO:

dimethylsulfoxide

EGTA:

ethyleneglycol-bis-(β-aminoethylether)-N,N,N′,N′-tetra acetic acid

FITC:

fluorescein isothiocyanate

PBS:

phosphate buffered saline

PIPES:

piperazine-1,4-bis-(2-ethanesulfonic acid)

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Authors and Affiliations

  1. Biologisches Institut der Universität Stuttgart, Pfaffenwaldring 57, D-70550, Stuttgart, Germany

    Ulrike Vallon & U. Kull

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  1. Ulrike Vallon
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  2. U. Kull
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Vallon, U., Kull, U. Localization of proteasomes in plant cells. Protoplasma 182, 15–18 (1994). https://doi.org/10.1007/BF01403684

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  • DOI: https://doi.org/10.1007/BF01403684

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