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The cyclic reorientation of cortical microtubules on walls with a crossed polylamellate structure: effects of plant hormones and an inhibitor of protein kinases on the progression of the cycle

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Summary

The outer tangential wall (OTW) of epidermal cells of azuki bean epicotyls has a crossed polylamellate structure, in which lamellae of longitudinal cellulose microfibrils alternate with lamellae of transverse cellulose microfibrils. This implies that the cyclic reorientation of cortical microtubules (MTs) from longitudinal to transverse and from transverse to longitudinal occurs on the OTW. Treatment with a solution that contained no auxin caused the accumulation of cells with longitudinal MTs, suggesting that auxin is required for the reorientation of MTs from longitudinal to transverse during the reorientation cycle. Treatment with 6-dimethylaminopurine (DMAP), an inhibitor of protein kinases that promoted the reorientation of MTs from transverse to longitudinal, resulted in the accumulation of cells with longitudinal MTs. Subsequent treatment with auxin caused a marked increase in the percentage of cells with transverse MTs and then a decrease in the percentage, indicating that the reorientation of MTs from longitudinal to transverse and then from transverse to longitudinal occurred during treatment with auxin. The percentage of cells with transverse MTs decreased more slowly in segments that had been pretreated with gibberellin A3 (GA) than in segments that had been pretreated without GA, suggesting that GA, in cooperation with auxin, caused the suppression of the reorientation of MTs from transverse to longitudinal.

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Abbreviations

BL:

brassinolide

BSA:

bovine serum albumin

GA:

gibberellin A3

DMAP:

6-dimethylaminopurine

DMSO:

dimethylsulfoxide

FITC:

fluorescein isothiocyanate

IAA:

indoleacetic acid

MT:

microtubule

OTW:

outer tangential wall

PBS:

phosphate-buffered saline

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Authors and Affiliations

  1. Department of Biology, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, 560, Osaka, Japan

    Keiko Mayumi & Hiroh Shibaoka

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  1. Keiko Mayumi
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  2. Hiroh Shibaoka
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Dedicated to Professor Eldon H. Newcomb in recognition of his contributions to cell biology

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Mayumi, K., Shibaoka, H. The cyclic reorientation of cortical microtubules on walls with a crossed polylamellate structure: effects of plant hormones and an inhibitor of protein kinases on the progression of the cycle. Protoplasma 195, 112–122 (1996). https://doi.org/10.1007/BF01279190

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  • DOI: https://doi.org/10.1007/BF01279190

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