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Localization of gene for human syndecan, an integral membrane proteoglycan and a matrix receptor, to chromosome 2

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Somatic Cell and Molecular Genetics

Abstract

Syndecan, a cell surface proteoglycan, is an integral membrane protein acting as a receptor for the extracellular matrix. For chromosomal localization of the human syndecan gene, a panel of mouse-human somatic cell hybrids was analyzed by Southern blotting using the cDNA probe for human syndecan. The hybrids were karyotyped at the time of DNA extraction. A band corresponding to the human syndecan gene in Southern blots was found only in a hybrid cell line containing human chromosome 2. This hybrid was subcloned and its subclones were analyzed by Southern blotting and karyotyped. Subclones carrying human chromosome 2 contained the syndecan gene, while subclones not carrying this chromosome did not. The human syndecan gene is thus assigned to chromosome 2.

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Authors and Affiliations

  1. Department of Genetics, University of Helsinki, Helsinki, Finland

    M. Ala-Kapee & J. Schröder

  2. Department of Obstetrics and Gynecology, University of Helsinki, Helsinki, Finland

    H. Nevanlinna

  3. Department of Medical Biochemistry, University of Turku, Turku, Finland

    M. Mali & M. Jalkanen

Authors
  1. M. Ala-Kapee
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  2. H. Nevanlinna
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  3. M. Mali
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  4. M. Jalkanen
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  5. J. Schröder
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Ala-Kapee, M., Nevanlinna, H., Mali, M. et al. Localization of gene for human syndecan, an integral membrane proteoglycan and a matrix receptor, to chromosome 2. Somat Cell Mol Genet 16, 501–505 (1990). https://doi.org/10.1007/BF01233200

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  • DOI: https://doi.org/10.1007/BF01233200

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