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Heterogeneous localization of some purine enzymes in subcellular fractions of rat brain and cerebellum

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Abstract

The activity of guanine deaminase (GAH, E.C. 3.5.4.3) was lower in rat cerebellum soluble and microsomal fractions than in rat brain subfractions. Adenosine deaminase (ADA, E.C. 3.5.4.4) activity was released in higher proportion than guanine deaminase, purine nucleoside phosphorylase (PNP, E.C. 2.1.2.4), 5′-nucleotidase (5′N, E.C. 3.1.3.5), and lactate (LDH, E.C. 1.1.1.27) and malate (MDH, E.C. 1.1.1.37) dehydrogenase in press-juices of rat brain. Furthermore, nerve ending-derived fractions (synaptosomes and synaptic vesicles) showed an enrichment of adenosine deaminase and also of 5′-nucleotidase. The action of deoxycholate over the subfractions did not increase the activity of either enzyme. The contrary occurred with the remaining enzymes studied. Thus, it is possible that one set of enzymes are located on the surface of the particulate vesicles, whereas another set are located inside these vesicles, suggesting a compartmentation of purine catabolic enzymes in different areas of the central nervous system.

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References

  1. Burnstock, G. 1975. Purine nucleotides and nucleosides as neurotransmitters or neuromodulators in the central nervous system. Pages 470–477.in Usdin, Hamburg, andBarchas (eds.), Neuroregulators and Psychiatric Disorders. Oxford University Press. New York.

    Google Scholar 

  2. Burnstock, G., andBrown, C. M., 1981. An introduction to purinergic receptors. Pages 1–45,in Burnstock, G. ed.). Purinergic Receptors (Receptors and Recognition. Series B, Vol. 12). pp. 1–45, Chapman and Hall. London.

    Google Scholar 

  3. Daly, J. W., Phillis, J. W., Kuroda, Y. andUi, M. (eds.) 1983. Physiology and Pharmacology of Adenosine Derivatives. Raven Press. New York.

    Google Scholar 

  4. Salganicoff, L., andDeRobertis, E., 1965). Subcellular distribution of the enzymes of the glutamic acid, glutamine, and γ-aminobutyric acid cycles in rat brain. J. Neurochem. 12:287–309.

    PubMed  Google Scholar 

  5. Burnstock, G. 1972. Purinergic nerves. Pharmacol. Rev. 24:509–581.

    PubMed  Google Scholar 

  6. Trams, E. G., andLauter, C. J. On the sidedness of plasma-membrane enzymes. Biochim. Biophys. Acta 345:180–197.

  7. Trams, E. G., andLauter, C. J., 1975. Adenosine deaminase in cultured brain cells. Biochem. J. 152:681–687.

    Google Scholar 

  8. Kai, M., White, G. L., andHawthorne, J. N., 1966. The phosphatidylinositol kinase of rat brain. Biochem. J. 101, 328–337.

    Google Scholar 

  9. Pull, I., andMcIlwain, H., 1974. Rat cerebral-cortex adenosine deaminase activity and its subcellular distribution. Biochem. J. 144:37–41.

    Google Scholar 

  10. Phillips, E., andNewsholme, E. A. 1979. Maximum activities, properties and distribution of 5′-nucleotidase, adenosine kinase and adenosine deaminase in rat and human brain. J. Neurochem. 33:553–558.

    PubMed  Google Scholar 

  11. Heymann, D., Reddington, M., andKreutzberg, G. W., 1984. Subcellular localization of 5′-nucleotidase in rat brain. J. Neurochem. 43:971–978.

    PubMed  Google Scholar 

  12. Kumar, S., Tewari, K., andKrishnan, P. S., 1965. Guanine deaminase activity in rat brain and liver. Biochem. J. 95:797–802.

    PubMed  Google Scholar 

  13. Amberson, W. R., Roisen, F. J., andBauer, A. C., The attachment of glycolytic enzymes to muscle ultrastructure. J. Cell Comp. Physiol. 66, 71–90.

  14. Franco, R., Canela, E. I., andBozal, J., 1986. Enzymes of the purine metabolism in rat brain microsomes. Neurochem. Res. 11:407–422.

    PubMed  Google Scholar 

  15. Mallol, J., andBozal, J., 1983. Modification of 5′-nucleotidase activity by divalent cations and nucleotides. J. Neurochem. 40:1205–1211.

    PubMed  Google Scholar 

  16. Tamir, H., Rapport, M. M., andRoizin, L., 1974. Preparation of synaptosomes and vesicles with sodium distrizoate. J. Neurochem. 23:943–949.

    PubMed  Google Scholar 

  17. Soller, M., Koenig, H., Milroie, R., Hughes, C., andLu, C. Y. 1973. Isolation and characterization of soluble acidic lipoproteins from rat brain synaptic vesicles. J. Neurochem, 21:557–572.

    PubMed  Google Scholar 

  18. Warburg, O., andChristian, W., 1942. Isolation and characterization of enolase. Biochem. Z. 310, 384-421. In accordance with a nomogram by Adams E. Cal. Corp. Biochem. Res.

  19. Lowry, O. H., Rosenbrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.

    PubMed  Google Scholar 

  20. Talwar, G. P., Goel, B. K., Mansoor, M., andPanda, N. C., 1961. Guanase activity in rat brain. J. Neurochem. 8:310–311.

    PubMed  Google Scholar 

  21. Mansoor, M., Talwar, G. P., andKalyanakar, G. D., 1963. Brain guanine deaminase purification, properties and regional distribution. Biochim. Biophys. Acta 77:307–317.

    PubMed  Google Scholar 

  22. Dawson, D. M. 1971. Absence of guanine deaminase from cerebellum. Neurology 21:621–626.

    PubMed  Google Scholar 

  23. Carpene, E., Falasca, A., Hakim, G., andSolaini, G., 1976. Distribution of guanase in some hog tissues. Bull. Soc. Ital. Biol. Sper. 52:497–502.

    Google Scholar 

  24. Tata, J. R. 1972. Preparation and properties of microsomal and submicrosomal fractions from secretory and non-secretory tissues. Pages 185–213,in Birnie, G. D. (ed.). Subcellular Components. Butterworths. London.

    Google Scholar 

  25. Stanley, K., Edwards, M. R., andLuzio, J. P., 1980. Subcellular distribution and movement of 5′-nucleotidase in rat cells. Biochem. J. 186:59–69.

    Google Scholar 

  26. Cammer, W., Sirota, S. R., Zimmerman, T. R., andNorton, W. T., 1980. 5′-nucleoti in rat brain myelin. J. Neurochem. 35:367–373.

    PubMed  Google Scholar 

  27. Depierre, J. W., andKarnowsky, 1974. Ecto-enzymes of the guinea pig polymorphonuclear leukocyte. I: Evidence for an ecto-adenosine monophosphatase,-adenosine triphosphatase and-p-nitrophenyl phosphatase. J. Biol. Chem. 249:7111–7120.

    PubMed  Google Scholar 

  28. Gurd, J. W., andEvans, W. H., 1974. Distribution of liver plasma-membrane 5′-Nucleotidase as indicated by its reaction with anti-plasma membrane serum. Arch. Biochem. Biophys. 164, 305–311.

    PubMed  Google Scholar 

  29. Newby, A. C., Luzio, J. P., andHales, C. N., 1975. The properties and extracellular localization of 5′-nucleotidase of the rat fat-cell plasma membrane. Biochem. J. 146:625–633.

    Google Scholar 

  30. Whittaker, V. P., Michaelson, I. A., andKirkland, R. J. A., 1964. The separation of synaptic vesicles from nerve-ending particles (‘synaptosomes’). Biochem. J. 90:293–303.

    PubMed  Google Scholar 

  31. Morgan, I. G., Vincendon, G., andGombos, G. 1973. Adult rat brain synaptic vesicles. I. Isolation and characterization. Biochim. Biophys. Acta 320:671–680.

    PubMed  Google Scholar 

  32. Widnell, C. C. 1972. Cytochemical localization of 5′-nucleotidase in subcellular fractions isolated from rat liver. I. Origin of 5′-nucleotidase activity in microsomes. J. Cell. Biol. 52:542–558.

    PubMed  Google Scholar 

  33. Andy, R. J. andKornfeld, R., 1982. The adenosine deaminase binding protein of human skin fibroblasts is located on the cell surface. J. Biol. Chem. 257, 7922–7925.

    PubMed  Google Scholar 

  34. Widnell, C. C. 1982. Evidence for a continual exchange of 5′-nucleotidase between the cell surface and cytoplasmic membranes in cultured rat fibroblasts. Cell 28:61–70.

    PubMed  Google Scholar 

  35. Lelievre, L., Zachowski, A., Maget-Dana, R., Aubry, J., andJonkman-Bark, G., 1977. Differences in the modulations of the soluble or plasma-membrane-bound 5′-nucleotidase. Eur. J. Biochem. 80, 185–191.

    PubMed  Google Scholar 

  36. Centelles, J. J., Franco, R., Canela, E. I., andBozal, J., 1985. Kinetics of the 5′-nucleotidase and the adenosine deaminase in subcellular fractions of rat brain. Neurochem. Res. Submitted.

  37. Scott, T. G. 1967. The distribution of 5′-nucleotidase in the rat brain of mouse. J. Comp. Neurol. 129:97–113.

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Bioquimica Fauultad de Quimica, Universidad de Barcelona, Diagonal 647, 08028, Barcelona, Catalunya, Spain

    Rafael Franco, Enrique I. Canela & Jorge Bozal

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  1. Rafael Franco
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  2. Enrique I. Canela
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Franco, R., Canela, E.I. & Bozal, J. Heterogeneous localization of some purine enzymes in subcellular fractions of rat brain and cerebellum. Neurochem Res 11, 423–435 (1986). https://doi.org/10.1007/BF00965016

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