Abstract
The structure of ribosomal DNA ofBrachypodium and several other grass species was investigated using a heterologous rDNA probe from wheat. Several different rDNA families were present among perennial and annual species within the genus. In contrast to the annual species the perennial species exhibited a very low degree of repeat length variation. An extra Eco RI site and a Hin dIII site were observed in the IGS, which distinguishedBrachypodium from other grass genera. The restriction fragment length polymorphism and length variation of the repeat units have taxonomic value withinBrachypodium and are correlated with the classification ofBrachypodium derived from other data.
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References
Appels, R., Dvorak, J., 1982: The wheat ribosomal DNA spacer region: its structure and variation in populations and among species. — Theor. Appl. Gen.63: 337–348.
—, 1986: rDNA: evolution over a billion years. InDutta, S. K., (Ed.): DNA systematics II, pp. 81–136. — Boca Raton, Florida: CRC Press.
Cordesse, F., Second, G., Delseny, M., 1990: Ribosomal gene spacer length variability in cultivated and wild rice species. — Theor. Appl. Gen.79: 81–88.
Doyle, J., Doyle, J. L., 1987: A rapid DNA isolation procedure for small quantities of fresh leaf tissue. — Phytochem. Bull.19: 11–15.
Ellis, T. H. N., Davies, D. R., Castleton, J. A., Bedford, I. D., 1984: The organisation and genetics of rDNA length variation in peas. — Chromosoma91: 74–81.
Feinberg, A. P., Vogelstein, B., 1983: A technique for radiolabelling DNA restriction fragments to high specific activity. — Ann. Biochem.163: 16–20.
Flavell, R. B., O'Dell, M., Sharp, P., Nevo, E., Beiles, A., 1986: Variation in the intergenic spacer of ribosomal DNA of wild wheatTriticum dicoccoides in Israel. — Mol. Biol. Evol.3: 547–558.
Gerlach, W. L., Bedbrook, J. R., 1979: Cloning and characterisation of ribosomal RNA genes from wheat and rye. — Nucl. Acid Res.7: 1869–1885.
Gill, B. S., Appels, R., 1988: Relationships between NOR-loci from differentTriticeae species. — Pl. Syst. Evol.160: 77–90.
Khan, M. A., 1984: Biosystematic studies inBrachypodium (Poaceae). — Ph. D. Thesis, University of Leicester.
Knaak, C., Hamby, R. K., Arnold, M. L., Leblanc, M. D., Chapman, R. L., Zimmer, E. A., 1990: Ribosomal DNA variation and its use in plant biosystematics. — InKawano, S., (Ed.): Biological approaches and evolutionary trends in plants, pp. 135–157. — London: Academic Press.
Long, E. O., David, I. B., 1980: Repeated genes in eukaryotes. — Ann. Rev. Biochem.49: 727–764.
Molnar, S. J., Fedak, G., 1989: Polymorphism in ribosomal DNA repeat units of 12Hordeum species. — Genome32: 1124–1127.
Reeder, R. H., 1984: Enhancers and ribosomal gene spacers. — Cell38: 349–351.
Rogers, S. O., Bendich, A. J., 1987: Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. — Pl. Mol. Biol.9: 509–520.
Saghai-Maroof, M. A., Soliman, K. M., Jongensen, R. A., Allard, R. W., 1984: Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosome location, and population dynamics. — Proc. Natl. Acad. Sci. USA81: 8014–8018.
Schippmann, U., 1991: Revision der europäischen Arten der GattungBrachypodium Palisot de Beauvois (Poaceae). — Boissiera45: 5–250.
Shi, Y., 1991: Molecular study of the genusBrachypodium (Poaceae). — Ph. D. Thesis, University of Leicester.
Springer, P., Zimmer, E. A., Bernetzen, J. L., 1989: Genomic organisation of the ribosomal DNA ofSorghum and its close relatives. — Theor. Appl. Gen.77: 844–850.
Tateoka, T., 1968: Phytogeographical notes on the genusBrachypodium P. Beauv. (Gramineae). — Bol. Soc. Argent. Bot.12: 44–56.
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Shi, Y., Draper, J. & Stace, C. Ribosomal DNA variation and its phylogenetic implication in the genusBrachypodium (Poaceae). Pl Syst Evol 188, 125–138 (1993). https://doi.org/10.1007/BF00937726
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DOI: https://doi.org/10.1007/BF00937726