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Release of 15-keto-13, 14-dihydro-thromboxane B2 and prostaglandin D2 during anaphylaxis as measured by radioimmunoassay

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Summary

  1. 1.

    A specific radioimmunoassy for 15-keto-13, 14-dihydro-thromboxane B2 was developed employing a polyvalent 125I-labelled tracer.

  2. 2.

    Using the radioimmunoassay large amounts of immunoreactive 15-keto-13, 14-dihydro-thromboxane B2 were found in perfusates of anaphylactic guinea pig lungs, but not in perfusates of anaphylactic guinea pig hearts. On the other hand, both lungs and hearts released immunoreactive thromboxane B2 after challenge, lungs being, however, much more active.

  3. 3.

    Using a radioimmunoassay for prostaglandin D2 variable amounts of this compound were detected in perfusates of anaphylactic guinea pig lungs.

  4. 4.

    While the cyclo-oxygenase inhibitor indomethacin (1 μg/ml) abolished release of thromboxane B2, 15-keto-13, 14-dihydro-thromboxane B2 and prostaglandins from anaphylactic guinea pig lungs, the thromboxane synthetase inhibitor imidazole (100 μg/ml) was without significant effect.

  5. 5.

    The results indicate that in anaphylactic guinea pig lungs, contrary to anaphylactic guinea pig hearts, a major fraction of the total thromboxane released into the perfusates is in the form of the biologically inactive 15-keto-13, 14-dihydro-thromboxane B2. On the other hand, prostaglandin D2, which can be formed enzymatically or non-enzymatically from the prostaglandin endoperoxide PGH2, might contribute to vascular and bronchial smooth muscle contraction observed in anaphylaxis.

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Authors and Affiliations

  1. Department of Pharmacology, Universität Freiburg, Hermann-Herder-Strasse 5, D-7800, Freiburg, Federal Republic of Germany

    H. Anhut, B. A. Peskar & W. Bernauer

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  1. H. Anhut
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  2. B. A. Peskar
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  3. W. Bernauer
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Anhut, H., Peskar, B.A. & Bernauer, W. Release of 15-keto-13, 14-dihydro-thromboxane B2 and prostaglandin D2 during anaphylaxis as measured by radioimmunoassay. Naunyn-Schmiedeberg's Arch. Pharmacol. 305, 247–252 (1978). https://doi.org/10.1007/BF00498818

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  • DOI: https://doi.org/10.1007/BF00498818

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