Skip to main content
Log in

Growth yield increase linked to caffeate reduction in Acetobacterium woodii

  • Original Papers
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Growth yields were determined with Acetobacterium woodii strain NZva 16 on hydrogen and CO2, formate, methanol, vanillate, ferulate and fructose in mineral medium in the absence and presence of 0.05% yeast extract. Yeast extract was not essential for growth but enhanced growth yields by 25–100% depending on the substrate fermented. Comparison of yields on formate or methanol allowed calculation of an energy yield in the range of 1.5–2 mol ATP per mol acetate formed during homoacetate fermentation of A. woodii. In the presence of 6 mM caffeate, growth yields were determined with the substrates formate or methanol. Caffeate was reduced to hydrocaffeate and increased growth yields were obtained. An ATP yield of about 1 mol per mol of caffeate reduced was calculated. Cytochromes were not detectable in cell free extracts or membrane preparations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Andreesen JR, Schaup A, Neurauter C, Brown A, Ljungdahl LG (1973) Fermentation of glucose, fructose, and xylose by Clostridium thermoaceticum: effect of metals on growth yield, enzymes, and the synthesis of acetate from CO2. J Bacteriol 114:743–751

    Google Scholar 

  • Bache R, Pfennig N (1981) Selective isolation of Acetobacterium woodi on methoxylated aromatic acids and determination of growth yields. arch Microbiol 130:255–261

    Google Scholar 

  • Braun K (1979) Untersuchungen zum autotrophen, heterotrophen und mixotrophen Wachstum von Acetobacterium woodi und Clostridium aceticum. Thesis, Univ. Göttingen

  • Diekert G, Ritter M (1982) Nickel requirement of Acetobacterium woodi. J Bacteriol 151:1043–1045

    Google Scholar 

  • Diekert G, Ritter M (1983) Carbon monoxide fixation into the carboxylgroup of acetate during growth of Acetobacterium woodi on H2 and CO2. FEMS Microbiol Lett 17:299–302

    Google Scholar 

  • Ljungdahl LG, Wood HG (1982) Acetate biosynthesis, In: Dolphin D (ed) B12, vol 2. John Wiley & Sons, Inc, New York, pp 165–202

    Google Scholar 

  • Pfennig N (1978) Rhodocyclus purpureus gen. nov, and sp. nov., a ring shaped, vitamin B12-requiring member of the family Rhodospirillaceae. Int J Syst Bacteriol 28:283–288

    Google Scholar 

  • Schink B, Pfennig N (1982) Fermentation of trihydroxybenzenes by Pelobacter acidigallici gen. nov. sp. nov., a new strictly anaerobic, non-sporeforming bacterium. Arch Microbiol 133: 195–201

    Google Scholar 

  • Singh AP, Bragg PD (1975) Reduced nicotinamid adenine dinucleotide dependent reduction of fumarate coupled to membrane energization in a cytochrome deficient mutant of Escherichia coli K12. Biochim Biophys Acta 396:229–241

    Google Scholar 

  • Stouthamer AH (1979) The search for correlation between theoretical and experimental growth yields. In: Quayle JR (ed) International review of biochemistry, microbial biochemistry, vol 21. University Park Press, Baltimore, pp 1–47

    Google Scholar 

  • Tanner R, wolfe RS, Ljungdahl LG (1978) Tetrahydrofolate enzyme levels in Acetobacterium woodi and their implication in the synthesis of acetate from CO2. J Bacteriol 134:668–670

    Google Scholar 

  • Winter J, Wolfe RS (1980) Methane formation from fructose by syntrophic associations of Acetobacterium woodii and different strains of methanogens. Arch Microbiol 124:73–79

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tschech, A., Pfennig, N. Growth yield increase linked to caffeate reduction in Acetobacterium woodii . Arch Microbiol 137, 163–167 (1984). https://doi.org/10.1007/BF00414460

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00414460

Key words

Navigation