Abstract
Thiosulfate-reductase activity (TSR) measured as sulfide release from thiosulfate was detected in crude extracts of Chlorella using dithioerythritol (DTE) as electron donor. Purification of this activity by ammonium-sulfate precipitation between 35% and 80% followed by Sephadex G-50 gel filtration, diethylaminoethyl-cellulose chromatography, and gel filtration on Biogel A 1.5 M led to four distinct proteins having molecular weights of: TSR I, 28000; TSR II, 26500; TSR IIIa, 55000; TSR IIIb, 24000 daltons. These thiosulfate reductases were most active with DTE; the monothiols glutathione, l-cysteine, and β-mercaptoethanol had little activity towards this system. The following pH optima were obtained: for TSR I and TSR II, 9.0; for TSR IIIa, 8.5; and for TSR IIIb, 9.5. The apparent-Km data for DTE and thiosulfate were determined to: \({\text{K}}_{{\text{m Na}}_{\text{2}} {\text{S}}_{\text{2}} {\text{O}}_{\text{3}} } \) TSR I, 0.164 mmol·l-1 and TSR II, 0.156 mmol·l-1; KmDTE TSR I, 1.54 mmol·l-1 and TSR II 1.54 mmol·l-1. The thiosulfate reductases IIIa and IIIb were further stimulated by addition of thioredoxin. All TSR fractions catalyzed SCN formation from thiosulfate and cyanate and thus had rhodanese activity; this activity, however, could only be detected in the presence of thiols.
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Abbreviations
- DTE:
-
dithioerythritol
- TSR:
-
thiosulfate reductase
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Dedicated to Professor Dr. Hubert Ziegler on the occasion of his 60th birthday
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Schmidt, A., Erdle, I. & Gamon, B. Isolation and characterization of thiosulfate reductases from the green alga Chlorella fusca . Planta 162, 243–249 (1984). https://doi.org/10.1007/BF00397446
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DOI: https://doi.org/10.1007/BF00397446