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Lateral hypothalamic lesions facilitate hepatic regeneration after partial hepatectomy in rats

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  • Molecular and Cellular Physiology
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Abstract

It has been reported that ventromedial hypothalamic lesions facilitate hepatic regeneration through the hepatic vagal nerve after partial hepatectomy. However, whether the lateral area of the hypothalamus is involved in liver regeneration after partial hepatectomy is unknown. To determine the role of the lateral hypothalamic area in this phenomenon, we studied hepatic DNA synthesis during liver regeneration after partial hepatectomy with bilateral lesions of the area. Lesioning of the lateral hypothalamus accelerated the increase in hepatic DNA synthesis and raised the peak level of [methyl-3H]thymidine incorporation after partial hepatectomy. These effects of hypothalamic lesioning were inhibited by combined hepatic vagotomy and sympathectomy. Our results demonstrate that lesioning of the lateral hypothalamus promotes hepatic regeneration through the autonomic nervous system after partial hepatectomy and suggest that the lateral hypothalamic area is involved in liver regeneration through neural mediation.

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References

  1. Ban T (1975) Fiber connections in the hypothalamus and some autonomic functions. Pharmacol Biochem Behav 3 [Suppl 1]:3–13

    Google Scholar 

  2. Bray GA, York DA (1979) Hypothalamic and genetic obesity in experimental animals: an autonomic and endocrine hypothesis. Physiol Rev 59:719–809

    Google Scholar 

  3. Bucher NLR, Swaffield MN (1975) Regulation of hepatic regeneration in rats by synergistic action of insulin and glucagon. Proc Natl Acad Sci USA 72:1157–1160

    Google Scholar 

  4. Burton K (1956) A study of the condition and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62:315–323

    Google Scholar 

  5. Cruise JL, Houck KA, Michalopoulos GK (1985) Induction of DNA synthesis in cultured rat hepatocytes through stimulation of alpha 1-adrenoreceptor by norepinephrine. Science 227:749–751

    Google Scholar 

  6. De Hemptinne B, Leffert HL (1983) Selective effects of portal blood diversion and glucagon on rat hepatocyte rates of S-phase entry and deoxyribonucleic acid synthesis. Endocrinology 112:1224–1232

    Google Scholar 

  7. Higgins GM, Andersen RM (1931) Experimental pathology of the liver. I. Restoration of the liver of the white rat following partial surgical removal. Arch Pathol 12:186–202

    Google Scholar 

  8. Holmin T, Ekelund M, Kullendorff C, Lindfeldt J (1984) A microsurgical method for denervation of the liver in the rat. Eur Surg Res 16:288–293

    Google Scholar 

  9. Iwai M, Shimazu T (1992) Alteration in sympathetic nerve activity during liver regeneration in rats after partial hepatectomy. J Auton Nerv Syst 41:209–214

    Google Scholar 

  10. Kiba T, Tanaka K, Hoshino M, Numata K, Inoue S (1993) Comparison of DNA synthesis of white and brown adipose tissue in rats with ventromedial hypothalamic lesions. Pflügers Arch 424:122–128

    Google Scholar 

  11. Kiba T, Tanaka K, Numata K, Hoshino M, Inoue S (1994) Facilitation of liver regeneration by ventromedial hypothalamic lesions after partial hepatectomy in rats. Pflügers Arch 428:26–29

    Google Scholar 

  12. Koch KS, Leffert HL (1979) Increased sodium ion influx is necessary to initiate rat hepatocyte proliferation. Cell 18:153–163

    Google Scholar 

  13. Lamar C Jr, Hollowly LS Jr. (1977) The effect of vagotomy on hepatic regeneration in rats. Acta Hepato-Gastroenterol 24:7–10

    Google Scholar 

  14. Leffert HL, Koch KS, Moran T, Rubaicava, B (1979) Humoral control of rat liver regeneration. Gastroenterology 76:1470–1482

    Google Scholar 

  15. Morley CGD, Royse VL (1981) Adrenergic agents as possible regulators of liver regeneration. Int J Biochem 13:969–973

    Google Scholar 

  16. Nakamura T, Nishizawa T, Hagiya M, Seki T, Shimonishi M, Sugumura A, Tashiro K, Shimizu S (1989) Molecular cloning and expression of human hepatocyte growth factor. Nature 342:440–443

    Google Scholar 

  17. Nakata M, Tsukamoto I, Nanme M, Makino S, Miyoshi M, Kojo S (1985) Alpha-adrenergic regulation of the activity of thymidylate synthetase and thymidine kinase during liver regeneration after partial hepatectomy. Eur J Pharmacol 114:355–360

    Google Scholar 

  18. Niijima A (1989) Nervous regulation of metabolism. Prog Neurobiol 33:135–147

    Google Scholar 

  19. Nobin A, Baumarten HG, Falck B, Ingemansson S, Moghimzadeh E, Rosengren E (1978) Organization of the sympathetic innervation in liver tissue from monkey and man. Cell Tissue Res 195:371–380

    Google Scholar 

  20. Oomura Y, Yoshimatsu H (1984) Neural network of glucose monitoring system. J Auton Nerv Syst 10:359–372

    Google Scholar 

  21. Paxinos G, Watson C (1982) The rat brain in stereotaxic coordinates. Academic, New York

    Google Scholar 

  22. Prechtl JC, Powley TL (1987) A light and electron microscopic examination of the vagal hepatic branch of the rat. Anat Embryol 176:115–126

    Google Scholar 

  23. Rogers RC, Hermann GE (1983) Central connections of the hepatic branch of the vagus nerve: a horseradish peroxidase histochemical study. J Auton Nerv Syst 7:165–174

    Google Scholar 

  24. Sakaguchi M, Takahashi M, Bray GA (1988) Lateral hypothalamus and sympathetic firing rates. Am J Physiol 255:R507-R512

    Google Scholar 

  25. Schneider WC (1946) Phosphorus compounds in animal tissues. III. A comparison of methods for the estimation of nucleic acids. J Biol Chem 164:747–751

    Google Scholar 

  26. Skaaring P, Bierring F (1976) On the intrinsic innervation of normal rat liver. Cell Tissue Res 171:141–155

    Google Scholar 

  27. Tanaka K, Ohkawa S, Nishino T, Niijima A, Inoue S (1987) Role of the hepatic branch of the vagus nerve in liver regeneration in rats. Am J Physiol 253:G439-G444

    Google Scholar 

  28. Yoshimatsu H, Niijima A, Oomura Y, Katafuchi T (1988) Lateral and ventromedial hypothalamic influences on hepatic autonomic nerve activity in the rat. Brain Res Bull 21:239–244

    Google Scholar 

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Kiba, T., Tanaka, K. & Inoue, S. Lateral hypothalamic lesions facilitate hepatic regeneration after partial hepatectomy in rats. Pflugers Arch. 430, 666–671 (1995). https://doi.org/10.1007/BF00386160

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  • DOI: https://doi.org/10.1007/BF00386160

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