Skip to main content
SpringerLink
Log in

Normal psoriatic epidermis expression of hyperproliferation-associated keratins

  • Original Contributions
  • Published:
Archives of Dermatological Research Aims and scope Submit manuscript

Summary

Keratin expression in lesional, marginal and uninvolved psoriatic epidermis was analysed by one- and two-dimensional gel electrophoresis and immunoblotting. Keratins K1, K5, K6, K10, K14, and K16 were identified in lesional epidermis. Keratins K6 and K16 were found in all epidermis probes of uninvolved skin, but never occurred in normal epidermis of control skin samples. By means of laser-densitometric evaluation of one-dimensional gels a downregulation of K1 and K10 and an upregulation of K6 and K16 was found in psoriatic epidermis. Unexpectedly, the level of K5 was considerably lower and the level of K14 considerably higher in lesional skin than in normal epidermis. These results demonstrate that not only basal keratinocytes in lesional epidermis but also suprabasal keratinocytes in uninvolved psoriatic epidermis express an altered differentiation pattern. The latter phenomenon could be very important in understanding the development of the so-called “Köbner effect” in psoriatic epidermis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Baden HP, Gilvray N, Cheng CK, Lee LD, Kubilus L (1978) The keratin polypeptides of psoriatic epidermis. J Invest Dermatol 70:294–297

    Google Scholar 

  2. Cooper D, Sun T-T (1986) Monoclonal antibody analysis of bovine epithelial keratins: specific pairs as defined by coexpression. J Biol Chem 261:4646–4654

    Google Scholar 

  3. De Mare S, Piet EJ, Erp BS van, Kerkhof PCM van de (1989) Epidermal hyperproliferation assessed by the monoclonal antibody Ks 8.12 on frozen sections. J Invest Dermatol 92:130–131

    Google Scholar 

  4. De Mare S, Jong E de, Erp PEJ van, Kerkhof PCM van de (1990) Markers for proliferation and keratinization in the margin of the active psoriatic lesion. Br J Dermatol 122:469–475

    Google Scholar 

  5. Eckert RL, Green H (1984) Cloning of cDNAs specifying vitamin A-responsive human keratins. Proc Natl Acad Sci USA 81:4321–4325

    Google Scholar 

  6. Eichner R, Bonitz P, Sun T-T (1984) Classification of epidermal keratins according to their immunoreactivity, isoelectric point and mode of expression. J Cell Biol 98:1388–1396

    Google Scholar 

  7. Eichner R, Sun T-T, Aebi U (1986) The role of keratin subfamilies and keratin pairs in the formation of human epidermal intermediate filaments. J Cell Biol 102:1767–1777

    Google Scholar 

  8. Fuchs E, Green H (1980) Changes in keratin gene expression during terminal differentiation of the keratinocyte. Cell 19:1033–1042

    Google Scholar 

  9. Fuchs E, Green H (1981) Regulation of terminal differentiation of cultured human keratinocytes by vitamin A. Cell 25:617–625

    Google Scholar 

  10. Fuchs E, Marchuk D (1983) Type I and Type II keratins have evolved from lower eukarytes to form the intermediate filaments in mammalian skin. Proc Natl Acad Sci USA 80:5857–5861

    Google Scholar 

  11. Fuchs E, Coppock S, Green H, Cleveland D (1981) Two distinct classes of keratin genes and their evolutionary significance. Cell 27:75–84

    Google Scholar 

  12. Gilfix BM, Green H (1984) Bioassay of retinoids using cultured human conjunctival keratinocytes. J Cell Physiol 119:172–174

    Google Scholar 

  13. Hatzfeld M, Maier G, Franke WW (1987) Cytokeratin domains involved in heterotypic complex formation determined by invitro binding assays. J Mol Biol 197:237–255

    Google Scholar 

  14. Holland DB, Wood EJ, Cunliffe WJ, Turner DM (1989) Keratin gene expression during the resolution of psoriatic plaques: effect of dithranol, PUVA, etretinate and hydroxyurea regimens. Br J Dermatol 120:9–19

    Google Scholar 

  15. Hunter I, Skerrow D (1981) The effect of increased tissue turnover on the keratinization of human epidermis. Biochem Biophys Acta 674:155–159

    Google Scholar 

  16. Huszar M, Gigi-Leistner O, Moll R, Franke WW, Geiger B (1986) Monoclonal antibodies to various acidic type cytokeratins of stratified epithelia. Differentiation 31:141–153

    Google Scholar 

  17. Kim KH, Rheinwald JG, Fuchs EV (1983) Tissue-specificity of epithelial keratins; differential expression of mRNAs from two multigene families. Mol Cell Biol 3:494–502

    Google Scholar 

  18. Kim KH, Schwartz F, Fuchs E (1984) Differences in keratin synthesis between normal epithelial cells and squamous cell carcinomas are mediated by vitamin A. Proc Natl Acad Sci USA 81:4280–4284

    Google Scholar 

  19. Kopan R, Fuchs E (1989) The use of retinoic acid to probe the relation between hyperproliferation-associated keratins and cell proliferation in normal and malignant epidermal cells. J Cell Biol 109:295–307

    Google Scholar 

  20. Kopan R, Traska G, Fuchs E (1987) Retinoids as important regulators of terminal differentiation: examining keratin expression in individual epidermal cells at various stages of keratinization. J Cell Biol 105:427–440

    Google Scholar 

  21. Korge B, Stadler R, Mischke D (1990) Effect of retinoids on hyperproliferation-associated keratins K6 and K16 in cultured human keratinocytes: a quantitative analysis. J Invest Dermatol 95:450–455

    Google Scholar 

  22. LÄmmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T-4. Nature 277:680–685

    Google Scholar 

  23. Le Vine MJ, McGilvray N, Baden HP (1980) Effect of therapy on keratin polypeptide profiles of psoriatic epidermis. Arch Dermatol 116:1028–1030

    Google Scholar 

  24. McGuire L, Osber M, Lightfoot L (1984) Two keratins MW 50000 and 56000 are synthesized by psoriatic epidermis. Br J Dermatol [Suppl 27]: 27–37

  25. Milstone LM, McGuire JJ (1981) Different polypeptides form the intermediate filaments in bovine hoof and esophageal epithelium and in aortic endothelium. J Cell Biol 88:312–316

    Google Scholar 

  26. Mischke D, Wild G (1987) Polymorphic keratins in human epidermis. J Invest Dermatol 88:191–197

    Google Scholar 

  27. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R (1982) The catalog of human epidermis cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24

    Google Scholar 

  28. Moll R, Moll I, Franke WW (1984) Differences of expression of cytokeratin polypeptides in various epithelial skin tumors. Arch Dermatol 276–363

  29. O'Farrell PZ, Goodmann HM, O'Farrell PH (1977) High resolution two dimensional electrophoresis of basic as well as of acidic proteins. Cell 12:1133–1142

    Google Scholar 

  30. Orfanos CE, Ehlert R, Gollnick H (1987) The retinoids: a review of their clinical pharmacology and therapeutic use. Drugs 34:459–503

    Google Scholar 

  31. Schiller DL, Franke WW, Geiger B (1982) Subfamily of relatively large and basic cytokeratin polypeptides as defined by peptide mapping is represented by one or several polypeptides in epithelial cells. EMBO J 1:761–769

    Google Scholar 

  32. Stadler R, Ingwerson A, Orfanos EC (1989) Immunotyping of the developing psoriatic plaque. J Cutan Pathol 16:291

    Google Scholar 

  33. Stadler R, Korge B, Müller K, Mischke D, Detmar M, Orfanos CE (1989) New potent arotinoid derivatives modify growth and differentiation of cultured murine and human keratinocytes. In: Reichert U, Shroot B (eds) Pharmacology of retinoids in the skin, vol 3. Karger, Basel, pp 15–19

    Google Scholar 

  34. Staquet MJ, Faure MR, Reano A, Viac J, Thivolet T (1983) Keratin polypeptides profile in psoriatic epidermis normalized by treatment with etretinate (aromatic retinoid Ro 10-9359). Arch Dermatol Res 275:124–129

    Google Scholar 

  35. Steinert MP, Roop DR (1988) Molecular and cellular biology of intermediate filaments. Ann Rev Biochem 57:593–625

    Google Scholar 

  36. Stoler A, Kopan R, Duvic M, Fuchs E (1988) Use of monospecific antisera and cRNA probes to localize the major changes in keratin expression during normal and abnormal epidermal differentiation. J Cell Biol 107:427–446

    Google Scholar 

  37. Sun T-T, Eichner R, Schermer A, Cooper D, Nelson WG, Weiss RA (1984) Classification, expression and possible mechanisms of evolution of mammalain keratins; an unifying model. In: Levine A, Topp W, Vande Wonde G, Watron JD (eds) Cancer cells (vol 1). The transformed phenotype. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 169–176

    Google Scholar 

  38. Thaler M, Fukuyama K, Epstein WL, Fisher KA (1980) Comparative studies of keratins isolated from psoriasis and atopic dermatitis. J Invest Dermatol 75:156–158

    Google Scholar 

  39. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc NatlAcad Sci USA 76:4350–4354

    Google Scholar 

  40. Tseng SCG, Jarvinen MJ, Nelson WG, Huang JN, Woodcock-Mitchell J, Sun T-T (1982) Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibodies study. Cell 30:361372

    Google Scholar 

  41. Tyner AL, Fuch E (1986) Evidence for posttranscriptional regulation of the keratins expressed during hyperproliferation and malignant transformation in human epidermis. J Cell Biol 103:1945–1955

    Google Scholar 

  42. Weiss RA, Eichner R, Sun T-T (1984) Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48 and 56 kdalton keratin as markers for hyperprqliferative keratinocytes. J Cell Biol 98:1397–1406

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Free University of Berlin, Germany

    M. Thewes

  2. Department of Dermatology, Medical Center Minden, Portastrasse 7-9, W-4950, Minden, Germany

    R. Stadler

  3. Department of Dermatology, Laboratory of Skin Biology, NIAMSD, Bethesda, USA

    B. Korge

  4. Institute for Experimental Oncology and Transplantation, University Center Charlottenburg, Germany

    D. Mischke

Authors
  1. M. Thewes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Stadler
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Korge
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. Mischke
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thewes, M., Stadler, R., Korge, B. et al. Normal psoriatic epidermis expression of hyperproliferation-associated keratins. Arch Dermatol Res 283, 465–471 (1991). https://doi.org/10.1007/BF00371784

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00371784

Key words

Search

Navigation