Abstract
A highly transcribed region in Oenothera mitochondria codes for an open reading frame comprising 250 condons (orf250). This open reading frame shows high sequence similarity to the helC gene of Rhodobacter capsulatus which encodes a subunit of a proposed ABC-type heme transporter. Transcripts of orf250 are edited by cytidine to uridine transitions at 29 sites, altering 10% of all encoded amino acids. Genes homologous to helC have also been found in the bacteria Bradyrhizobium japonicum and Escherichia coli, and are conserved in mitochondria of Marchantia polymorpha, Daucus carota, and Arabidopsis thaliana. In bacteria these genes belong to operons that are involved in the biogenesis of c-type cytochromes. The bacterial gene organization is partly conserved in Marchantia, but altered in the mitochondrial genome of Oenothera.
Similar content being viewed by others
References
Ames GF-L (1986) Bacterial periplasmic transport systems: Structure, mechanism, and evolution. Annu Rev Biochem 55:397–425
Beckman DL, Kranz RG (1993) Cytochrome c biogenesis in a photosynthetic bacterium requires a periplasmic thioredoxin-like protein: Proc Natl Acad Sci USA 90 2179–2183
Beckman DL, Trawick DR, Kranz RG (1992) Bacterial cytochrome c biogenesis. Genes Dev 6 268–283
Blattner FR, Burland V, Plunkett III G, Sofia HJ, Daniels DL (1993) Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res 21:5408–5417
Braun HP, Emmermann M, Kruft V, Schmitz UK (1992) Cytochrome c, 1,from potato: a protein with a presequence for targeting to the mitochondrial intermembrane space. Mol Gen Genet 231:217–225
Covello PS, Gray MW (1989) RNA editing in plant mitochondria. Nature 341:662–666
Dewey RE, Levings III CS, Timothy DH (1986) Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell 44:439–449
Gonzalez DH, Bonnard G, Grienenberger J-M (1993) A gene involved in the biogenesis of cytochromes is co-transcribed with a ribosomal protein gene in wheat mitochondria. Curr Genet 24:248–255
Gualberto JM, Lamattina L, Bonnard G, Weil J-H, Grienenberger J-M (1989) RNA editing in wheat mitochondria results in the conservation of protein sequences. Nature 341:660–662
Hiesel R, Wissinger B, Schuster W, Brennicke A (1989) RNA editing in plant mitochondria. Science 246:1632–1634
Higgins CF (1992) ABC transporters: From microorganisms to man. Ann Rev Cell Biol 8:67–113
Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun C-R, Meng B-Y, Li Y-Q, Kanno A, Nishizawa Y, Hirai A, Shinizaki K, Sugiura S (1989) The complete sequence of the rice (Oryza sativa) chloroplast genome: intramolecular recombinations between distinct tRNA genes account for a major plastid DNA inversion during the evolution of the cereals. Mol Gen Genet 217:185–194
Hyde SC, Emsley P, Hartshorn MJ, Mimmack MM, Gileadi U, Pearce SR, Gallagher MP, Gill DR, Hubbard RE, Higgins CF (1990) Structural model of ATP-binding proteins associated with cystic fibrosis, multidrug resitence and bacterial transport. Nature 346:362–365
Kyle J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105–132
Lipman DJ, Pearson WR (1985) Rapid and sensitive similarity searches. Science 227:1435–1441
Nakazono M, Hirai A (1993) Identification of the entire set of transferred chloroplast DNA sequences in the mitochondrial genome of rice. Mol Gen Genet 236:341–346
Nicholson DW, Neupert W (1989) Import of cytochrome c into mitochondria: reduction of heme, mediated by NADH and flavin nucleotides, is obligatory for its covalent linkage to apocytochrome c. Proc Natl Acad Sci USA 86:4340–4344
Nicholson DW, Stuart RA, Neupert W (1989) Biogenesis of cytochrome c 1 .J Biol Chem 264:10156–10168
Oda K, Yamato K, Ohta E, Nakamura Y, Takemura M, Nozato N, Akashi K, Kanegae T, Ogura Y, Kohchi T, Ohyama K (1992) Gene organization deduced from the complete sequence of liverwort Marchantia polymorpha mitochondrial DNA. J Mol Biol 223:1–7
Ohyama K, Fukuzawa H, Kochi T, Shira H, Sano T, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Chloroplast genome organization deduced from complete sequence of liverwort Marchantia polymorpha chloroplast DNA. Nature 322:572–574
Pearson WR, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444–2448
Poole RK, Hatch L, Cleeter MWJ, Gibson F, Cox GB, Wu G (1993) Cytochome bd biosynthesis in Escherichia coli: the sequence of the cycC and cycD genes suggests that they encode the components of an ABC membrane transporter. Mol Microbiol 10:421–430
Pritchard AE, Scilhamer JJ, Mahalingam CL, Sable CL, Venuti SE, Cummings DJ (1990) Nucleotide sequence of the mitochondrial genome of Paramecium. Nucleic Acids Res 18:173–180.
Ramseier TM, Winteler HV, Hennecke H (1991) Discovery and sequence analysis of bacterial genes involved in the biogenesis of c-type cytochromes. J Biol Chem 266:7793–7803
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Sanger F, Nicklen S, Coulson A (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Schuster W (1994) The highly edited orf206 in Oenothera mitochondria may encode a component of a heme transporter involved in cytochrome c biogenesis. Plant Mol Biol 25:33–42
Schuster W, Hiesel R, Wissinger B, Schobel W, Brennicke A (1989) Isolation and analysis of plant mitochondria and their genomes. In: Shaw CH (ed) Plant molecular biology. IRL Press, Oxford, Washington DC, pp 79–1020
Schuster W, Combettes B, Flieger K, Brennicke A (1993) A plant mitochondrial gene encodes a protein involved in cytochrome c biogenesis. Mol Gen Genet 239:49–57
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shonozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Neno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO J 5:2043–2049
Valentin K, Maid U, Emrich A, Zetsche K (1992) Organization and expression of a phycobiliprotein gene cluster from the unicellular alga Cyanidium caldarium. Plant Mol Biol 20:267–276
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jekabsons, W., Schuster, W. orf250 encodes a second subunit of an ABC-type heme transporter in Oenothera mitochondria . Molec. Gen. Genet. 246, 166–173 (1995). https://doi.org/10.1007/BF00294679
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00294679