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Regulation of cessation of respiration and killing by cyclic 3′,5′-adenosine monophosphate and its receptor protein after far-ultraviolet irradiation of Escherichia coli

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Summary

When Escherichia coli B/r cultures are irradiated with ultraviolet light (UV) (254 nm), those cells that are killed stop respiring by 60 min after irradiation. Post-UV treatment with cyclic adenosine 3′,5′-adenosine monophosphate (cAMP) causes more cells to stop respiring and to die. We have studied these effects at a UV fluence of 52 J/m2 in a wild-type E. coli K12 strain and in mutants defective in cAMP metabolism. Strain CA 8000 has crp + and cya + genes for the cAMP receptor protein (CRP) (required for transcription of operons regulated by cAMP) and for adenylate cyclase, respectively; CA 7901 is crp -; and CA 8306 is a cya deletion (Δ). The wild-type culture showed a small transient cessation of respiration, and addition of cAMP caused cessation to be nearly complete. The crp - culture showed no evidence of cessation of respiration, and cAMP had no effect. The Δcya mutant also showed no cessation of respiration, but cAMP (5 mM) caused as complete inhibition as in the wild type. cAMP caused a 10-fold loss in viability of UV-irradiated wild-type and Δcya liquid cultures but had no effect on the cpr - culture. Respiration and viability changes were also studied in a double mutant, CA8404 Δcya crp *, which has an altered CRP that is, with respect to the lac operon, independent of cAMP. The respiration response to UV was similar to that of the wild-type culture, and both respiration and viability of cells in liquid culture were sensitive to cAMP. The survival data, obtained by plating immediately after irradiation, show the wild type, Δcya strains, and Δcya crp * to be equally sensitive and the crp - strain to be more resistant. We conclude that cessation of respiration and cell killing after UV irradiation are regulated by cAMP and the CRP.

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Authors and Affiliations

  1. Biology Division, Oak Ridge National Laboratory, 37830, Oak Ridge, Tennessee

    P. A. Swenson & R. L. Schenley

  2. Biochemistry Department, The University of Tennessee, 37916, Knoxville, Tennessee, USA

    J. G. Joshi

Authors
  1. P. A. Swenson
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  2. J. G. Joshi
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  3. R. L. Schenley
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Communicated by B.A. Bridges

Research supported by the Energy Research and Development Administration under contract with the Union Carbide Corporation, and by grant no. PCM76-80294 from The National Science Foundation to J.G.J.

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Swenson, P.A., Joshi, J.G. & Schenley, R.L. Regulation of cessation of respiration and killing by cyclic 3′,5′-adenosine monophosphate and its receptor protein after far-ultraviolet irradiation of Escherichia coli . Molec. Gen. Genet. 159, 125–130 (1978). https://doi.org/10.1007/BF00270885

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  • DOI: https://doi.org/10.1007/BF00270885

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