Abstract
The accumulation of abundant proteins and their respective transcripts, induced by 10−4 M cisabscisic acid or 10−5 M jasmonic acid methyl ester, was studied in barley (Hordeum vulgare L.) leaf segments and compared to that resulting from osmotic stress caused by floating the segments on solutions of sorbitol, glucose, polyethyleneglycol (PEG)-6000 or NaCl. Osmotic stress or treatment with abscisic acid led to the synthesis of novel proteins which were identical to jasmonateinduced proteins (JIPs) with respect to immunological properties and molecular masses. The most prominent polypeptides were characterized by molecular masses of 66, 37 and 23 kDa and were newly synthesized. Whereas sorbitol, mannitol, sucrose, glucose and PEG provoked the synthesis of JIPs, 2deoxyglucose and NaCl did not. We provide evidence that the synthesis of JIPs induced by osmotic stress is directly correlated with a preceding rise in endogenous jasmonates. These jasmonates, quantified by an enzyme immunoassay specific for (−)jasmonic acid and its aminoacid conjugates, increased remarkably in leaf segments treated with sorbitol, glucose or other sugars. In contrast, no increase in jasmonates could be observed in tissues exposed to salts (NaCl). The results strengthen the hypothesis that the accumulation of jasmonates, probably by de-novo synthesis, is an intermediate and essential step in a signalling pathway between (osmotic) stress and activation of genes coding for polypeptides of high abundance.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ABA cis :
-
abscisic acid
- JA:
-
jasmonic acid
- JIP:
-
jasmonic acid-induced protein
- JM:
-
jasmonic acid methylester
- PEG:
-
polyethyleneglycol
- Pin:
-
proteinase inhibitor
References
Andresen, I., Becker, W., Schlüter, K., Burges, J., Parthier, B., Apel, K. (1992) The identification of leaf thionin as one of the main jasmonate-induced proteins of barley (Hordeum vulgare). Plant Mol. Biol. 19, 193–204
Bartels, D., Schneider, K., Terstappen, G., Piatkowski, D., Salamini, F. (1990) Molecular cloning of abscisic acid-modulated genes which are induced during desiccation of the resurrection plant Craterostigma plantagineum. Planta 181, 27–34
Bell, E., Mullet, J.E. (1991) Lipoxygenase gene expression is modulated in plants by water deficit, wounding, and methyl jasmonate. Mol. Gen. Genet. 230, 456–462
Creelman, R.A., Tierney, M.L., Mullet, J.E. (1992) Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. Proc. Natl. Acad. Sci. USA 89, 4938–4941
Esen, A. (1978) A simple method for quantitative, semiquantitative, and qualitative assay of protein. Anal. Biochem. 89, 264–273
Grimes, H.D., Tranbarger, T.J., Franceschi, V.R. (1993) Expression and accumulation patterns of nitrogen-responsive lipoxygenase in soybeans. Plant Physiol. 103, 457–466
Gundlach, H., Müller, M.J., Kutchan, T.M., Zenk, M.H. (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc. Natl. Acad. Sci. USA 89, 2389–2393
Herrmann, G., Lehmann, J., Peterson, A., Sembdner, G., Weidhase, R.A., Parthier, B. (1989) Species and tissue specificity of jasmonate-induced abundant proteins. J. Plant Physiol. 134, 703–709
Hildmann, Th., Ebneth, M., Peña-Cortés, H., Sánchez-Serrano, J.J., Willmitzer, L. (1992) General roles of abscisic and jasmonic acids in gene activation as a result of mechanical wounding. Plant Cell 4, 1157–1170
Johnson, R., Ryan, C.A. (1990) Wound-inducible potato inhibitor II genes: enhancement of expression by sucrose. Plant Mol. Biol. 14, 527–536
Kim, S.-R., Choi, J.-L., Costa, M.A., An, G. (1992) Identification of G-box sequence as an essential element for methyl jasmonate response of potato proteinase inhibitor II promoter. Plant Physiol. 99, 627–631
Knöfel, H.-D., Brückner, C., Kramell, R., Sembdner, G., Schreiber, K. (1990) Radioimmunoassay for the natural plant growth regulator (−)-jasmonic acid. Biochem. Physiol. Pflanz. 186, 387–394
Mason, H.S., Mullet, J.E. (1990) Expression of two soybean vegetative storage protein genes during development and in response to water deficit, wounding, and jasmonic acid. The Plant Cell 2, 569–579
Mason, H.S., De Wald, D.B., Creelman, R.A., Mullet, J.E. (1992) Coregulation of soybean vegetative storage protein gene expression by methyl jasmonate and soluble sugars. Plant Physiol. 98, 859–867
Mason, H.S., De Wald, D.B., Mullet, J.E. (1993) Identification of a methyl jasmonate-responsive domain in soybean vsp B promoter. Plant Cell 5, 241–251
Melan, M.A., Dong, X., Endara, M.E., Davis, K.R., Ausubel, F.M., Peterman, T.K. (1993) An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate. Plant Physiol. 101, 441–450
Meyer, A., Miersch, O., Büttner, C., Sembdner, G., Schreiber, K. (1984) Occurrence of the plant growth regulator jasmonic acid in plants. J. Plant Growth Regul. 3, 1–8
Mueller-Uri, F., Parthier, B., Nover, L. (1988) Jasmonate-induced alteration of gene expression in barley leaf segments analyzed by in vivo and in vitro synthesis. Planta 176, 241–247
Mundy, J., Chua, N.-H. (1988) Abscisic acid and water-stress induce the expression of a novel rice gene. The EMBO J. 7, 2279–2286
O'Farrell, P.H. (1975) High-resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007–4021
Parthier, B. (1991) Jasmonates, new regulators of plant growth and development: Many facts and few hypotheses on their actions. Bot. Acta 104, 446–454
Parthier, B., Brückner, C., Dathe, W., Hause, B., Herrmann, G., Knoefel, D., Kramel, H.-D., Kramel, R., Lehmann, J., Miersch, O., Reinbothe, S., Sembdner, G., Wasternack, C., Zur Nieden, U. (1992) Jasmonates: Metabolism, biological activities, and modes of action in senescence and stress responses. In: Progress in plant growth regulation, pp. 276–285, Karssen, C.M., Van Loon, L.C., Vreugdenhil, D., eds. Kluwer Acad. Publ. Dordrecht
Reinbothe, S., Machmudowa, A., Wasternack, C., Reinbothe, C., Parthier, B. (1992a) Jasmonate-induced proteins in cotton; immunological relationship to the respective barley proteins and homology of transcripts to late embryogenesis abundant (Lea) mRNAs. J. Plant Growth Regul. 11, 7–14
Reinbothe, S., Reinbothe, C., Lehmann, J., Parthier, B. (1992b) Differential accumulation of methyl Jasmonate-induced mRNAs in response to abscisic acid and desiccation in barley (Hordeum vulgare). Physiol. Plant. 86, 49–56
Reinbothe, S., Reinbothe, C., Lehmann, J., Becker, W., Apel, K., Parthier, B. (1994) JIP60, a methyl jasmonate-induced ribosome-inactivating protein involved in plant stress reactions. Proc. Natl. Acad. Sci. USA 91, 7012–7016
Ryan, C.A. (1992) The search for the proteinase inhibitor-inducing factor PIIF. Plant Mol. Biol. 19, 123–133
Sembdner, G., Parthier, B. (1993) Biochemistry, physiological and molecular actions of Jasmonates. Annu. Rev. Plant Physiol. 44, 569–589
Singh, N.K., Nelson, D.E., Kuhn, D., Hasegawa, P.M., Bressan, R.A. (1989) Molecular cloning of osmotin and regulation of its expression by ABA and adaptation to low water potential. Plant Physiol. 90, 1096–1101
Skriver, K., Mundy, J. (1990) Gene expression in response to abscisic acid and osmotic stress. Plant Cell 2, 503–512
Staswick, P.E., Huang, J.F., Thee, Y. (1991) Nitrogen and methyl jasmonate induction of soybean vegetative storage protein genes. Plant Phisiol. 96, 130–136
Towbin, H., Staehlin, T., Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc. Natl. Acad. Sci. USA 76, 4350–4354
Wasternack, C., Atzorn, R., Blume, B., Leopold, J., Parthier, B. (1994) Ursolic acid inhibits synthesis of jasmonate-induced proteins in barley leaves. Phytochemistry 35, 49–54
Weidhase, R.A., Lehmann, J., Kramell, H.M., Sembdner, G., Parthier, B. (1987a) Degradation of ribulose-1,5-bisphosphate carboxylase and chlorophyll in senescing barley leaf segments triggered by jasmonic acid methyl ester, and counteraction by cytokinin. Physiol. Plant. 69, 161–166
Weidhase, R.A., Kramell, H.M., Lehmann, J., Liebisch, W., Lerbs, W., Parthier, B. (1987b) Methyljasmonate-induced changes in the polypeptide pattern of senescing barley leaf segments. Plant Sci. 51, 177–186
Weiler, E.W. (1986) Plant hormone immunoassays based on monoclonal and polyclonal antibodies. In: Modern methods of plant analysis, vol. 4: Immunology in plant sciences, pp. 1–17, Linskens, H.F., Jackson, J.F., eds. Springer, Berlin Heidelberg New York Tokyo
Wilen, R.W., Van Rooijen, G.J.H., Pearcce, D.W., Pharis R.P., Holbrook, L.A., Moloney, M.M. (1991) Effects of jasmonic acid on embryo-specific processes in Brassica and Linum oilseeds. Plant Physiol. 95, 399–405
Author information
Authors and Affiliations
Additional information
Dedicated to Professor Dr. Peter Sitte, Freiburg i. Br., on the occasion of his 65th birthday
This work was in part supported by a grant from the Deutsche Forschungsgemeinschaft (SFB 363). The authors thank B. Degner, C. Gebhardt, K. Manke, and R. Weiß for their technical assistance.
Rights and permissions
About this article
Cite this article
Lehmann, J., Atzorn, R., Brückner, C. et al. Accumulation of jasmonate, abscisic acid, specific transcripts and proteins in osmotically stressed barley leaf segments. Planta 197, 156–162 (1995). https://doi.org/10.1007/BF00239952
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00239952