Abstract
A double-1RS wheat-rye translocation line lacking all B subunits of glutenin was produced in durum wheat cv ‘Langdon’ for use in backcrosses and testcrosses in the study of the inheritance of low-molecular-weight (LMW) glutenin subunits in tetraploid wheats. The B subunits of glutenin and γ-and ω-gliadin bands present in parents derived from Triticum durum and T. dicoccoides, encoded by Glu-3 and Gli-1 loci, respectively, were found to be inherited mainly as units (blocks), as reported previously. Two rare recombination events between the Glu-A3 and Gli-A1 loci were detected in testcross progeny from ‘Edmore’ x T. dicoccoides landrace 19–27. Several rare recombinants were also detected within the 1BS-controlled B subunits of glutenin blocks, suggesting that there are two separate tightly linked loci (3.07±1.35 cM) within the Glu-B3 ‘locus’. Evidence was also obtained for the presence of an additional locus coding for a B subunit of glutenin in ‘Edmore’ that is loosely linked (20.9±3.18%) with the main Glu-B3 ‘locus’.
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Bushuk W, Zillman RR (1978) Wheat cultivar identification by gliadin electrophoregrams. I. Apparatus, method and nomenclature. Can J Plant Sci 58:505–515
Colot V, Bartels D, Thompson R, Flavell R (1989) Molecular characterization of an active wheat LMW glutenin gene and its relation to the other wheat and barley prolamin genes. Mol Gen Genet 216:81–90
Galili G, Feldman M (1984) Mapping of glutenin and gliadin genes located on chromosome 1B of common wheat. Mol Gen Genet 193:293–298
Gupta RB (1989) Low-molecular-weight subunits of glutelin in wheat and related species: their characterization, genetics and relation to bread-making quality. PhD thesis, The University of Adelaide, Australia
Gupta RB, Shepherd KW (1988) Low-molecular-weight glutenin subunits in wheat: their variation, inheritance and association with bread-making quality. In:Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp. Bath Press, Bath, England, pp 943–949
Gupta RB, Shepherd KW (1993) Production of multiple wheat-rye 1RS translocation stocks and genetic analysis of LMW subunits of glutenin and gliadins in wheats using these stocks. Theor Appl Genet 85:719–728
Hanson WD (1959) Minimum family sizes for the planning of genetic experiments. Agron J 51:711–716
Jackson EA, Holt LM, Payne PI (1985) Glu-B2, a storage protein locus controlling the D group of LMW glutenin subunits in bread wheat (Triticum aestivum). Genet Res 46:11–17
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Lawrence GJ, Shepherd KW (1981) Inheritance of glutenin protein subunits of wheat. Theor Appl Genet 60:333–337
Liu C-Y (1995) Identification of a new low Mr glutenin subunit locus on chromosome 1B of durum wheat. J Cereal Sci 22 (in press)
Liu C-Y, Shepherd KW (1991) Variation of LMW glutenin subunits in durum wheat cultivars associated with quality. In:Martin DJ, Wrigley CW (eds) Proc Conf Cereals Int. Cereal Chemistry Division, Royal Australian Chemical Institute, Victoria, Australia, pp 263–266
Mather K (1951) The measurement of linage in heredity. Methuen's monographs on biological subjects, 2nd edn., Methuen, London
Metakovsky EV, Akhmedov MG, Sozinov AA (1986) Genetic analysis of gliadin-encoding genes reveals gene clusters as well as single remoted genes. Theor Appl Genet 73:278–285
Metakovsky EV, Wrigley CW, Bekes F, Gupta RB (1990) Gluten polypeptides as useful genetic markers of dough quality in Australian wheats. Aust J Agric Res 41:289–306
Nevo E, Beiles A (1989) Genetic diversity of wild emmer wheat in Israel and Turkey. Structure, evolution, and application in breeding. Theor Appl Genet 77:421–455
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Physiol 38:141–153
Payne PI, Holt LM, Lawrence GJ, Law CN (1982) The genetics of gliadin and glutenin, the major storage proteins of the wheat endosperm. Qual Plant-Plant Fd Hum Nutr 31:229–241
Payne PI, Jackson EA, Holt LM (1984) The association between γ-gliadin 45 and gluten strength in durum wheat varieties: a direct causal effect or the result of genetic linkage? J Cereal Sci 2:73–81
Payne PI, Holt LM, Lister PG (1988) Gli-A3 and Gli-B3, two newly designated loci coding for omega-type gliadins and D subunits of glutenin. In:Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp. Bath Press, Bath, England, pp 999–1002
Pogna NE, Lafiandra D, Feillet P, Autran J-C (1988) Evidence for a direct causal effect of low-molecular-weight subunits of glutenins on gluten viscoelasticity in durum wheats. J Cereal Sci 7:211–214
Pogna NE, Autran J-C, Mellini G, Lafiandra D, Feillet P (1990) Chromosome 1B-encoded gliadins and glutenin subunits in durum wheat:genetics and relationship to gluten strength. J Cereal Sci 11:15–34
Pogna NE, Metakovsky EV, Redaelli R, Raineri F, Dachkevitch T (1993) Recombination mapping of Gli-5, a new gliadin-coding locus on chromosomes 1A and 1B in common wheat. Theor Appl Genet 87:113–121
Redaelli R, Pogna NE, Dachkevitch T, Cacciatori P, Cancardi AM, Metakovsky EV (1992) Inheritance studies of the 1AS/1DS chromosome translocation in bread wheat variety ‘Perzivan-1’. J Genet Breed 46:253–262
Ruiz M, Carrillo JM (1993) Linkage relationships between prolamin genes on chromosomes 1A and 1B of durum wheat. Theor Appl Genet 87:353–360
Singh NK, Shepherd KW (1988) Linkage mapping of the genes controlling endosperm proteins in wheat. 1. Genes on the short arms of group 1 chromosome. Theor Appl Genet 75:628–641
Singh NK, Shepherd KW, Cornish GB (1991) A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. J Cereal Sci 14:203–208
Sobko TA (1984) Identification of a locus controlling the synthesis of endosperm alcohol-soluble proteins in winter bread wheat (in Ukrainian). J Agri Sci 7:78–80
Sozinov AA, Poperelya FA (1982) Polymorphism of prolamines and variability of grain quality. Qual Plant-Plant Foods Hum Nutr 31:243–249
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Communicated by F. Salamini
Portion of this paper was presented at the 8th Int Wheat Genet Symp in Beijing, China, August 1993
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Liu, CY., Shepherd, K.W. Inheritance of B subunits of glutenin and ω-and γ-gliadins in tetraploid wheats. Theoret. Appl. Genetics 90, 1149–1157 (1995). https://doi.org/10.1007/BF00222936
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DOI: https://doi.org/10.1007/BF00222936