Summary
Ribophorin I and II (RPN I and RPN II), two specific glycoproteins, span the rough regions of the endoplasmic reticulum (RER) and are thought to play an important role either in translocation or in the maintenance of RER. Studies with human-mouse somatic cell hybrids have localized the gene for RPN I on human chromosome 3q, while RPN II is on chromosome 20. Using a radioactive labelled cDNA probe, we have regionally mapped the RPN II gene to human chromosome 20q12-q13.1 by in situ hybridization. This assignment predicts a location of the murine homologue, Rpn-2, to the syntenic segment on mouse chromosome 2 in close proximity to Ada, Src and Gnas.
References
Barton DE, Crimaudo C, Hortsch M, Franke U (1987) The genes for ribophorin I and II are on human chromosome 3q and 20 and mouse chromosomes 6 and 2, respectively. Cytogenet Cell Genet 46:557
Beermann F, Hummler E, Franke U, Hansmann I (1988) Allocation of a transgenic c-myc integration site to mouse chromo-some 8B3-C1. Cytogenet Cell Genet 49:311–312
Chandler ME, Yunis JJ (1978) A high resolution in-situ hybridization technique for the direct visualization of labelled G-banded early metaphase and prophase chromosomes. Cytogenet Cell Genet 22:352–356
Crimaudo C, Hortsch M, Gausepohl H, Meyer DI (1987) Human ribophorins I and II: the primary structure and membrane topology of two highly conserved rough endoplasmic reticulumspecific glycoproteins. EMBO J 6:75–82
Kreibich G, Ulrich BL, Sabatini DD (1978a) Proteins of rough microsomal membranes related to ribosome binding. I. Identification of ribophorin I and II, membrane proteins characteristic of rough microsomes. J Cell Biol 77:464–487
Kreibich G, Freienstein CM, Pereyra BN, Ulrich BL, Sabatini DD (1978b) Proteins of rough microsomal membranes related to ribosome binding. II. Cross-linking of bound ribosomes to specific membrane proteins expose at the binding sites. J Cell Biol 77:488–506
Lyon MF, Kirby MC (1990) Mouse chromosome atlas. Mouse Genome 87:28–50
Redman CM (1969) J Biol Chem 244:4308–4315
Rosenfeld MG, Marcantonio EE, Hakimi J, Ort VM, Atkinson PH, Sabatini DD, Kreibich G (1984) Biosynthesis and processing of ribophorins in the endoplasmic reticulum. J Cell Biol 99:1076–1082
Rybak J, Tharapel S, Garcia M, Maniken C, Freeman M (1982) A simple reproducible method for prometaphase chromosome analysis. Hum Genet 60:328–333
Yoshida H, Tondokoro N, Asano Y, Mizusawa K, Yamagishi R, Horigome T, Sugano H (1987) Studies on membrane proteins involved in ribosome binding on the rough endoplasmic reticulum. Biochem J 245:811–819
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Löffler, C., Rao, V.V.N.G. & Hansmann, I. Mapping of the ribophorin II (RPN II) gene to human chromosome 20q12–q13.1 by in-situ hybridization. Hum Genet 87, 221–222 (1991). https://doi.org/10.1007/BF00204188
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00204188