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Autoradiographic studies on the role of plasmodesmata in the transport of gibberellin

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Abstract

Translocation of [14C]gibberellic acid into antheridial cells of Chara vulgaris L. was investigated in relation to the presence of symplasmic connections between the antheridium and the thallus. It was found that manubria, capitular cells, and antheridial filaments were about three-fold more strongly labelled in young antheridia connected to the thallus by plasmodesmata than in older antheridia in which spontaneous symplasmic isolation had occurred. Plasmolytically induced symplasmic isolation of young antheridia severely diminished the radioactivity of all the cells, down to the level characteristic for spontaneously isolated antheridia. It is concluded that plasmodesmata are the main channel of gibberellin transport into antheridia. The change in the character of symplasmic connections during the course of morphogenesis might, among other events, constitute a signal determining a shift of cell metabolism in a new direction, in response to a rapid change in gibberellin level.

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Abbreviations

GA(n) :

gibberellin (An)

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

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Authors and Affiliations

  1. Laboratory of Cytophysiology, University of Łódź, ul., Banacha 12/16, Pl-90 237, Łódź, Poland

    Maria Kwiatkowska

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  1. Maria Kwiatkowska
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This study was supported by the Polish Academy of Sciences research project CPBP 04.01.5.05.

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Kwiatkowska, M. Autoradiographic studies on the role of plasmodesmata in the transport of gibberellin. Planta 183, 294–299 (1991). https://doi.org/10.1007/BF00197801

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