Abstract
Growth of 293 cells (human embryonic kidney) was compared in various cell culture systems including static flasks, cell aggregates and a variety of porous microcarriers. The best results were achieved with Fibra-Cel carriers and cell aggregates (1.1–1.4 × 106 cells/ml). Virus production was compared using a recombinant replication-deficient adenovirus as a model. Virus yields of lysates from cells grown on Fibra-Cel carriers, as cell aggregates and in static flasks were comparable (1.7–1.9 × 108 pfu/ml).
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Racher, A.J., Fooks, A.R. & Griffiths, J.B. Culture of 293 cells in different culture systems: Cell growth and recombinant adenovirus production. Biotechnol Tech 9, 169–174 (1995). https://doi.org/10.1007/BF00157073
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DOI: https://doi.org/10.1007/BF00157073