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Microbial utilization of coral mucus in long term in situ incubation over a coral reef

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Abstract

The purpose of this study was to assess degradation and utilization of the mucus produced by 3 coral reef Anthozoa (Sarcophyton, Fungia and Acropora) by microorganisms. This was achieved by carrying out long term in situ incubations at Nouméa lagoon (New Caledonia).

The microbial population including bacterial and eukaryotic cells was monitored by cell counts, cultures of mucus degraders, and by estimation of microbial activity from the pool of adenylates and enzymatic activity. In addition the chemical composition (C and N) of the mucus was monitored and its morphological features were observed by scanning electron microscope (SEM).

Only slight differences were found between the 3 types of mucus studied. On the whole, they follow the same pattern of change. After a short bacterial growth phase (4 days), a bloom of eukaryotes (Flagellates, Ciliates and Diatoms) was observed. This eukaryote population remained constant for at least 10 days. A similar pattern has been described in the breakdown of detritus of plant origin.

Several observations suggest that bacteria utilize only certain components of mucus, the most widely used being proteins, triglycerides and wax esters; these latter two compounds are known to be the dissolved photosynthetic products released by zooxanthellae during mucus secretion. Neither bacteria nor eukaryotes completely degrade the mucus web even after 21 days of incubation. The likelihood that mucus excretion is a defensive reaction against physical and chemical stresses might explain why mucus is a poor, or even inhibiting medium for the bacterial degraders isolated from the mucus itself.

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References

  • Benson, A. A., L. Muscatine, 1974. Wax in coral mucus: Energy transfer from corals to reef fishes. Limnol. Oceanogr. 19: 810–814.

    Google Scholar 

  • Coffroth, M. A., 1988. The role and fate of mucous sheet produced by reef coelenterates. In Proc. 6th int. coral Reef Congr. Townsville, abstr.

  • Coles, S. L., R. Strathmann, 1973. Observations on coral mucus ‘flocs’ and their potential trophic significance. Limnol. Oceanogr. 18: 673–678.

    Google Scholar 

  • Coll, J. C., B. F. Bowden, D. M. Tapiolas, 1982. In situ isolation of allelo-chemicals released from soft corals (Coelenterata: Octocorallia): a totally submersible sampling apparatus. J. Exp. Mar. Biol. Ecol. 60: 293–299.

    Article  Google Scholar 

  • Crossland, C. J., 1987. In situ release of mucus and DOC-lipid from the corals Acropora variabilis and Stylophora pistillata in different light regimes. Coral Reefs 6: 35–42.

    Article  Google Scholar 

  • Crossland, C. J., D. J. Barnes, M. A. Borowitzka, 1980. Diurnal lipid and mucus production in the Staghorn Coral Acropora acuminata. Mar. Biol. 60: 81–90.

    Article  Google Scholar 

  • Daumas, R., B. A. Thomassin, 1977. Protein fractions in coral and zoantharian mucus: possible evolution in coral reef environments. In Proc. 3rd int. Coral Reef Symp., Rosenstiel School of Marine and Atmospheric Science, Univ. Miami, Miami, U.S.A.: 517–523.

    Google Scholar 

  • Daumas, R., R. Galois, B. A. Thomassin, 1982. Biochemical composition of soft and hard coral mucus on a new caledonian lagoonal reef. In Proc. 4th int. Coral Reef Symp., Univ. Philippines, Manila, 1981, 2: 59–67.

    Google Scholar 

  • Ducklow, H. W., R. Mitchell, 1979a. Composition of mucus released by coral reef coelenterates. Limnol. Oceanogr. 24: 706–714.

    Google Scholar 

  • Ducklow, H. W., R. Mitchell, 1979b. Bacterial populations and adaptations in the mucus layers on living corals. Limnol. Oceanogr. 24: 715–725.

    Google Scholar 

  • Ferguson, R. L., E. N. Buckley, A. V. Palumbo, 1984. Response of marine bacterioplankton to differencial filtration and confinement. Appl. Envir. Microbiol. 47: 49–55.

    Google Scholar 

  • Herndl, G. J., B. Velimirov, 1986. Microheterotrophic utilization of mucus released by the Mediterranean coral Cladocora cespitosa. Mar. Biol. 90: 363–369.

    Article  Google Scholar 

  • Humble, M. W., A. King, I. Phillips, 1977. APIZYM: a simple rapid system for detection of bacterial enzymes. J. Clin. Pathol. 30: 275–277.

    PubMed  Google Scholar 

  • Johannes, R. E., 1967. Ecology of organic aggregates in the vicinity of a coral reef. Limnol. Oceanogr. 12: 189–195.

    Google Scholar 

  • Karl, M., O. Holm-Hansen, 1978. Methodology and measurement of adenylate energy charge ratios in environmental samples. Mar. Biol. 48: 185–197.

    Article  Google Scholar 

  • Kato, M., 1987. Mucus-sheet formation and discoloration in the reef-building coral, Porites cylindrica: effects of altered salinity and temperature. Galaxea 6: 1–16.

    Google Scholar 

  • Krupp, D. A., 1982. The composition of the mucus from the mushroom coral, Fungia scutaria. In Proc. 4th int. Coral Reef Symp., Univ. Philippines, Manila, 1981 2: 69–73

    Google Scholar 

  • Krupp, D. A., 1984. Mucus production by corals exposed during an extreme low tide. Pac. Sci. 38: 1–11.

    Google Scholar 

  • La Barre, S. C., J. C. Coll, P. W. Sammarco, 1986. Competitive strategies of soft corals (Coelenterata: octocorallia): 3 spacing and aggressive interactions between alcyonaceans. Mar. Ecol. Prog. Ser. 28: 147–156.

    Google Scholar 

  • Landry, M. R., L. N. Haass, V. L. Fagerness, 1984. Dynamics of microbial plankton communities: experiments in Kaneohe Bay, Hawaï. Mar. Ecol. Prog. Ser. 16: 127–133.

    Google Scholar 

  • Linley, E. A. S., R. C. Newell, S. A. Bosma, 1981. Heterotrophic utilization of mucilage released during fragmentation of kelp (Ecklonia maxima and Laminaria pallida). I. Development of microbial communities associated with the degradation of kelp mucilage. Mar. Ecol. Prog. Ser. 4: 31–41.

    Google Scholar 

  • Lynch, J. M., N. J. Poole, 1980. Microbial Ecology. A conceptual approach. Blackwell Scientific Publ. 266 pp.

  • Marshall, N., 1973. Notes on mucus and zooxanthellae discharged from reef corals. Proc. 1st int. Symp. Corals and coral reefs, Mandapain Comp., 12–16 January 1969, p. 59–65.

  • Means, J. C., A. C. Sigleo, 1986. Contribution of coral reef mucus to the colloidal organic pool in the vicinity of Discovery Bay, Jamaica, W.I. Bull. mar. Sci. 39: 110–118.

    Google Scholar 

  • Meikle, P., G. N. Richards, D. Yellowlees, 1988. Structural investigations on the mucus from six species of coral. Mar. Biol. 99: 187–193.

    Article  Google Scholar 

  • Monget, D., 1978. Mise au point d'une microméthode de détection et de mesure d'activités enzymatiques (APIZYM). Résultats obtenus dans différents domaines d'application. Thése Docteur Ingénieur, Univ. Lyon.

  • Moriarty, D. J. W., P. C. Pollard, W. G. Hunt, 1985a. Temporal and spatial variation in bacterial production in the water column over a coral reef. Mar. Biol. 85: 285–292.

    Article  Google Scholar 

  • Moriarty, D. J. W., P. C. Pollard, D. M. Alongi, C. R. Wilkinson, J. S. Gray, 1985b. Bacterial productivity and trophic relationships with consumers on a coral reef (MECOR 1). In: Proc. 5th int. Coral Reef Congr., E.P.H.E., Tahiti 3: 457–462.

    Google Scholar 

  • Muscatine, L., 1973. Nutrition of corals. In Jones O. A., Endean R. (eds). Biology and geology of coral reefs, Vol. 2: Biology, Academic Press, New-York: 77–115.

    Google Scholar 

  • Newell, R. C., E. A. S. Linley, M. I. Lucas, 1983. Bacterial production and carbon conversion based on salt marsh plant debris. Estuar. coast. Shelf Sci. 17: 405–419.

    Article  Google Scholar 

  • Pascal, H., 1981. Valeur nutritive des mucus produits par deux anthozoaires récifaux du golfe d'Aqaba (Mer Rouge) vis à vis des populations bactériennes. Thèse 3ème cycle, Fac. Sci. Marseille-Luminy, Univ. Aix-Marseille II, 112 pp.

  • Pascal, H., E. Vacelet, 1981. Bacterial utilization of mucus on the coral reef of Aqaba (Red Sea). In Proc. 4th int. Coral Reef Symp., Univ. Philippines, Manila, 1: 669–677.

    Google Scholar 

  • Patton, J. S., S. Abraham, A. A. Benson, 1977. Lipogenesis in the intact coral Pocillopora capitata and its isolated zooxanthellae: evidence for a light-driven carbon cycle between symbiont and host. Mar. Biol. 44: 235–247.

    Article  Google Scholar 

  • Patton, J. S., J. F. Battey, M. W. Rigler, J. W. Porter, C. C. Black, J. E. Burris, 1983. A comparison of the metabolism of bicarbonate 14C and acetate 1−14C and the variability of species lipid compositions in reef corals. Mar. Biol. 75: 121–130.

    Article  Google Scholar 

  • Paul, J. H., M. F. DeFlaun, W. H. Jeffrey, 1986. Elevated levels of microbial activity in the coral surface microlayer. Mar. Ecol. Prog. Ser. 33: 29–40.

    Google Scholar 

  • Richards, G. N., P. J. Meikle, D. Yellowless, 1983. Preliminary investigations into the chemical nature of mucus from the staghorn coral (Acropora formosa). In Proc. Great Barrier Reef inaugural Conf., Townsville, Aug. 29–2 Sept. 1983, ed. Baker et al., 353–359.

  • Richman, S., Y. Loya, L. B. Slobodkin, 1975. The rate of mucus production by corals and its assimilation by the coral reef copepod Acartia negligens. Limnol. Oceanogr. 20: 918–923.

    Google Scholar 

  • Rublee, P. A., H. R. Lasker, M. Gottfried, M. R. Roman, 1980. Production and bacterial colonization of mucus from the soft coral Briarium asbestinum. Bull. mar. Sci. 30: 888–893.

    Google Scholar 

  • Sammarco, P. W., J. C. Coll, S. La Barre, 1985. Competitive strategies of soft corals (Coelenterata: Octocorallia). II. Variable defensive responses and susceptibility to scleractinian corals. J. Exp. Mar. Biol. Ecol. 91: 199–215.

    Article  Google Scholar 

  • Vacelet, E., 1985. Quelques exemples d'utilisation de l'APIZYM en écologie. Rapp. Comm. int. Mer. Médit. 29: 23–24.

    Google Scholar 

  • Vacelet, E., B. A. Thomassin, 1985. Utilization of mucus of some coral species of New Caledonia by bacteria in in situ experiments. In Proc. 5th int. Coral Reef Congr., E.P.H.E., Tahiti: 2: 385.

    Google Scholar 

  • Wiebe, W. J., L. R. Pomeroy, 1972. Microorganisms and their association with aggregates and detritus in the sea: a microscopic study. In Melchiorri-Santolini U., Hopton J. W. (eds) ‘Detritus and its role in aquatic ecosystems’. Proc. IBP-UNESCO Symp., Mem. Ist. Ital. Idrobiol. 29 (suppl.): 325–352.

  • Wipple, C. E., 1901. Changes that take place in bacterial contents of waters during transportation. Technol. Q. Proc. Soc. Arts 14: 21–29.

    Google Scholar 

  • Witzel, K. P., 1979. The adenylate energy charge as a measure of microbial activities in aquatic habitats. Arch. Hydrobiol., Beih. Ergebn. Limnol. 12: 146–165.

    Google Scholar 

  • Zimmerman, R., 1977. Estimation of bacterial number and biomass by epifluorescence microscopy and scanning electron microscopy. In G. Rheinheimer (ed.) ‘Microbial Ecology of a brackish water environment’. Ecological Studies 25, Springer Verlag: 103.

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  1. Centre d'Océnologie de Marseille, Station marine d'Endoume, rue de la Batterie des Lions, 13007, Marseille, France

    Eveline Vacelet & Bernard A. Thomassin

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  1. Eveline Vacelet
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  2. Bernard A. Thomassin
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Vacelet, E., Thomassin, B.A. Microbial utilization of coral mucus in long term in situ incubation over a coral reef. Hydrobiologia 211, 19–32 (1991). https://doi.org/10.1007/BF00008613

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  • DOI: https://doi.org/10.1007/BF00008613

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