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Improvement of CPP-RBD for siRNA Delivery

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Animal Cell Technology: Basic & Applied Aspects

Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 16))

Abstract

Small interfering RNA (siRNA) causes sequence-specific gene silencing. In this study, siRNAs were induced into the cells by using a cell-penetrating peptide (CPP) and RNA-binding protein (RBP) as a safe and effective siRNA carrier (CPP-RBP). The diffusion of the siRNAs into the cytoplasm and RNAi mediated gene silencing were induced by photostimulation of Alexa546, which was connected to the CPP-RBP. Here, we investigated the effects of several CPP-RBPs for effective inducing the RNAi.

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References

  1. Endoh, T., Sisido, M., and Ohtsuki, T. (2008) Cellular siRNA delivery mediated by a cell-permeant RNA-binding protein and photoinduced RNA interference. Bioconjug. Chem. 19: 1017.

    Article  CAS  PubMed  Google Scholar 

  2. Handa, N., Nureki, O., Kurimoto, K., Kim, I., Sakamoto, H., Shimura, Y., Muto, Y. and Yokoyama, S. (1999) Structural basis for recognition of the tra mRNA precursor by the Sex-lethal protein. Nature 398: 579.

    Article  CAS  PubMed  Google Scholar 

  3. Scharpf, M., Sticht, H., Schweimer, K., Boehm, M., Hoffmann, S., and Rosch, P. (2000) Antitermination in bacteriophage lambda. The structure of the N36 peptide-boxB RNA complex. Eur. J. Biochem. 267: 2397.

    Article  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan

    Rina Kuwabara, Tamaki Endoh, Masahiko Sisido & Takashi Ohtsuki

  2. Department of Bioscience and Biotechnology, Okayama University, Okayama, Japan

    Masahiko Sisido & Takashi Ohtsuki

Authors
  1. Rina Kuwabara
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  2. Tamaki Endoh
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  3. Masahiko Sisido
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  4. Takashi Ohtsuki
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Corresponding author

Correspondence to Rina Kuwabara .

Editor information

Editors and Affiliations

  1. Fac. Engineering, Dept. Chemical Engineering, Kyushu University, Motooka 744, Fukuoka, 819-0395, Japan

    Masamichi Kamihira

  2. Fac. Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan

    Yoshinori Katakura

  3. Fac. Engineering, Kyushu University, Fukuoka, 819-0395, Japan

    Akira Ito

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© 2010 Springer Science+Business Media B.V.

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Kuwabara, R., Endoh, T., Sisido, M., Ohtsuki, T. (2010). Improvement of CPP-RBD for siRNA Delivery. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_31

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