Abstract
Enzymes from ectothermic organisms are adapted to function within specific niches in the environment. There seems to be parity in respect of conformational stability, flexibility and activity for enzymes at the growth temperature for the source organisms.1 This makes cold-adapted enzymes particularly suited for catalysis at low temperatures. Organic phase biocatalysis (OPB) refers to the performance of enzymatic reactions in media comprising wholly or partly of organic solvents. This approach extends the well-known advantages of enzymatic catalysis (e.g., high specificity and catalytic efficiency, mild reaction conditions) to reactions involving water insoluble substrates. Replacing an aqueous reaction medium with an organic solvent allows novel reactions, e.g. reverse hydrolysis and transesterification, to be carried out. OPB grew rapidly in the mid-1980’s with applications of enzymes, whole cells and tissues, as biocatalysts in water-organic solvent phase systems.
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Apenten, R.K.O. (1999). Low temperature organic phase biocatalysis using cold-adapted enzymes. In: Margesin, R., Schinner, F. (eds) Biotechnological Applications of Cold-Adapted Organisms. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58607-1_3
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DOI: https://doi.org/10.1007/978-3-642-58607-1_3
Publisher Name: Springer, Berlin, Heidelberg
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