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MicroRNA Profiling Using RNA from Microdissected Immunostained Tissue

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Laser Capture Microdissection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 755))

Abstract

mRNA expression profiling has been used to define molecular subtypes of human breast cancer. Also microRNAs have been investigated in these breast cancer subtypes. However, little is known regarding the microRNA signature of healthy luminal and basal breast epithelial cells. Therefore, a method is described to isolate immunostained luminal and basal breast epithelial cells in formalin-fixed paraffin-embedded tissues by laser microdissection. Employing this new methodological approach, we could identify distinct microRNA profiles of luminal and basal breast epithelial cells by real-time PCR-based profiling.

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Authors and Affiliations

  1. Institute of Pathology, Medizinische Hochschule Hannover, Hannover, Germany

    Clemens L. Bockmeyer, Danny Jonigk, Hans Kreipe & Ulrich Lehmann

Authors
  1. Clemens L. Bockmeyer
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  2. Danny Jonigk
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  3. Hans Kreipe
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  4. Ulrich Lehmann
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Corresponding author

Correspondence to Ulrich Lehmann .

Editor information

Editors and Affiliations

  1. , Department of Pathology, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

    Graeme I. Murray

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© 2011 Springer Science+Business Media, LLC

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Bockmeyer, C.L., Jonigk, D., Kreipe, H., Lehmann, U. (2011). MicroRNA Profiling Using RNA from Microdissected Immunostained Tissue. In: Murray, G. (eds) Laser Capture Microdissection. Methods in Molecular Biology, vol 755. Humana Press. https://doi.org/10.1007/978-1-61779-163-5_7

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  • DOI: https://doi.org/10.1007/978-1-61779-163-5_7

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-162-8

  • Online ISBN: 978-1-61779-163-5

  • eBook Packages: Springer Protocols

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