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3D Cell Culture pp 129–153Cite as

Production of Tissue-Engineered Skin and Oral Mucosa for Clinical and Experimental Use

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 695))

Abstract

Since the early 1990s, our understanding of how epithelial and stromal cells interact in 3D tissue-engineered constructs has led to tissue-engineered skin and oral mucosa models, which are beginning to deliver benefit in the clinic (usually in small-scale reconstructive surgery procedures) but have a great deal to offer for in vitro investigations. These 3D tissue-engineered models can be used for a wide variety of purposes such as dermato- and mucotoxicity, wound healing, examination of pigmentation and melanoma biology, and in particular, a recent development from this laboratory, as a model of bacterially infected skin. Models can also be used to investigate specific skin disease processes. In this chapter, we describe the basic methodology for producing 3D tissue-engineered skin and oral mucosa based on de-epidermised acellular human dermis, and we give examples of how these models can be used for a variety of applications.

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Acknowledgements

We gratefully acknowledge the continued support for our research throughout the years of the Burns and Plastic Surgery Consultants of the Northern General Hospital Trust Burns Unit, particularly Mr Eric Freedlander and Mr David Ralston. We also acknowledge the histopathology services provided over the years by Dr Chris Layton – ever helpful.

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Authors and Affiliations

  1. Department of Materials Science and Engineering, Kroto Research Institute, University of Sheffield, Sheffield, UK

    Sheila MacNeil, Joanna Shepherd & Louise Smith

Authors
  1. Sheila MacNeil
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  2. Joanna Shepherd
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  3. Louise Smith
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Editors and Affiliations

  1. Kroto Research Institute, Department of Engineering Materials, University of Sheffield, Broad Lane, Sheffield, S3 7HW, United Kingdom

    John W. Haycock

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MacNeil, S., Shepherd, J., Smith, L. (2011). Production of Tissue-Engineered Skin and Oral Mucosa for Clinical and Experimental Use. In: Haycock, J. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 695. Humana Press. https://doi.org/10.1007/978-1-60761-984-0_9

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  • DOI: https://doi.org/10.1007/978-1-60761-984-0_9

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-983-3

  • Online ISBN: 978-1-60761-984-0

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