Abstract
Natural-killer (NK) cells play an important role in recognizing and eliminating virally infected and transformed cells. To study this process, convenient assays for NK-cell function are required. Conventional NK-cell activity assays measure the release of 51Cr from prelabeled target cells following membrane disruption. This chapter describes nonradiometric assays for NK-cell killing of adenovirus-transformed human cells that can be applied to multiple cell samples using flow cytometry.
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© 2007 Humana Press Inc, Totowa, NJ
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Bottley, G., Cook, G.P., Blair, G.E. (2007). A Flow Cytometric Assay for Analysis of Natural-Killer Cell-Mediated Cytolysis of Adenovirus-Transformed Cells. In: Wold, W.S.M., Tollefson, A.E. (eds) Adenovirus Methods and Protocols. Methods in Molecular Medicine™, vol 131. Humana Press. https://doi.org/10.1007/978-1-59745-277-9_16
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DOI: https://doi.org/10.1007/978-1-59745-277-9_16
Publisher Name: Humana Press
Print ISBN: 978-1-58829-901-7
Online ISBN: 978-1-59745-277-9
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