Short Communication
Rapid assay for detection of methicillin-resistantStaphylococcus aureususing multiplex PCR

https://doi.org/10.1006/mcpr.1996.0066Get rights and content

Abstract

The presence or absence of themecAgene, the determinant of resistance to all β-lactam antibiotics, was examined in clinical isolates ofStaphylococcus aureusby multiplex polymerase chain reaction (MPCR). Two pairs of primers were used, which yielded two specific products; a 280-bpnuc-based PCR fragment (amplification product of thenucgene encoding specificStaphylococcus aureusnuclease) and a 533-bpmecA-based PCR fragment (amplification product of themecAgene). The MPCR system was designed to be incorporated into the work flow in clinical diagnostic laboratories as a routine analysis.

References (0)

Cited by (79)

  • Coagulase-negative staphylococci (CoNS) isolated from ready-to-eat food of animal origin - Phenotypic and genotypic antibiotic resistance

    2015, Food Microbiology
    Citation Excerpt :

    For all the tet(M)-positive isolates the presence of conjugative transposons of the Tn916–Tn1545 family was determined by using primers targeting the integrase gene int according to Doherty et al. (2000). Detection of mecA gene were carried out according to protocols described by Barski et al. (1996). Detection of erm(A), erm(C) and mrs(A/B) gene were carried out according to Sutcliffe et al. (1996).

  • Discrimination of methicillin-resistant Staphylococcus aureus from methicillin-susceptible Staphylococcus aureus or coagulase-negative staphylococci by detection of penicillin-binding protein 2 and penicillin-binding protein 2' using a bioluminescent enzyme immunoassay

    2013, Journal of Immunological Methods
    Citation Excerpt :

    Some strains with borderline or low levels of resistance have also been found to not produce PBP2′ (Hiramatsu et al., 1992). Although polymerase chain reaction (PCR) can detect the mecA gene encoding PBP2′, the potential for cross contamination, long turnaround times, the expense and availability of specialized equipment and trained staff may deter some laboratories from using PCR in clinical diagnosis (Barski et al., 1996; Bignardi et al., 1996; Murakami et al., 1991). Furthermore, some strains carrying the mecA gene may not exhibit methicillin resistance (Tokue et al., 1992).

View all citing articles on Scopus
f1

Author to whom correspondence should be addressed.

View full text