Short CommunicationRapid assay for detection of methicillin-resistantStaphylococcus aureususing multiplex PCR
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Molecular Identification and Genotyping of Staphylococci: Genus, Species, Strains, Clones, Lineages, and Interspecies Exchanges
2018, Pet-to-Man Travelling Staphylococci: A World in ProgressCoagulase-negative staphylococci (CoNS) isolated from ready-to-eat food of animal origin - Phenotypic and genotypic antibiotic resistance
2015, Food MicrobiologyCitation Excerpt :For all the tet(M)-positive isolates the presence of conjugative transposons of the Tn916–Tn1545 family was determined by using primers targeting the integrase gene int according to Doherty et al. (2000). Detection of mecA gene were carried out according to protocols described by Barski et al. (1996). Detection of erm(A), erm(C) and mrs(A/B) gene were carried out according to Sutcliffe et al. (1996).
Discrimination of methicillin-resistant Staphylococcus aureus from methicillin-susceptible Staphylococcus aureus or coagulase-negative staphylococci by detection of penicillin-binding protein 2 and penicillin-binding protein 2' using a bioluminescent enzyme immunoassay
2013, Journal of Immunological MethodsCitation Excerpt :Some strains with borderline or low levels of resistance have also been found to not produce PBP2′ (Hiramatsu et al., 1992). Although polymerase chain reaction (PCR) can detect the mecA gene encoding PBP2′, the potential for cross contamination, long turnaround times, the expense and availability of specialized equipment and trained staff may deter some laboratories from using PCR in clinical diagnosis (Barski et al., 1996; Bignardi et al., 1996; Murakami et al., 1991). Furthermore, some strains carrying the mecA gene may not exhibit methicillin resistance (Tokue et al., 1992).
Current molecular approach for diagnosis of MRSA: a meta-narrative review
2022, Drug Target Insights
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