Modelling Complex Populations Formed by Proliferating, Quiescent and Quasi-quiescent Cells: Application to Plant Root Meristems

Abstract

A proliferating population of cells may be considered complex when its proliferative or growth fractionP is lower than 1 and/or when it is formed by subpopulations with different mean cycle times. The present paper shows that in such complex populations exponential growth is consistent with a steady-state distribution of cells. Obviously, whenP =1 then cell distribution is only a function of cell age. An analytical model has been developed to study complex populations including both quiescent fractions formed by cells with unreplicated genome (G₀ cells) and cells with fully duplicated chromosomes (Q₂ cells). The model also considers those quasi-quiescent cells in their last transit through G₁ and S (Q₁ and Q_s cells) before becoming quiescent. In order to solve the difficulties of a direct analysis of the whole population, its kinetic parameters have been obtained by studying the negative exponential distribution of two subpopulations: one formed by the proliferating cells and another formed by the quasi-quiescent cells. Additionally, the model could be applied when quiescence is initiated at any other cycle phase different from G₁ and G₂, for instance, cells in the process of replicating their DNA or being at any other mitotic phases. The utility of the method was illustrated in populations which constitute the root meristems of both Allium cepa L. and Pisum sativum L. Three facts should be stressed: (1) the method seems to be rather powerful because it can be carried out from different sets of experimentally measured parameters; (2) the rate of division and, therefore, the population doubling time can be easily estimated by this method; and (3) it also allows the determination of the amount of cells that had become quiescent either before they had replicated their DNA (G₀) or after having completed their replication (Q₂), as well as those quasi-quiescent cells which are progressing throughout their last pre-replicative and replicative periods (thus Q₁ and Q_s, respectively).