Regular Article
The Replication of Autographa californica Baculovirus (AcMNPV) in Two Lepidopteran Cell Lines Grown in Serum-Free Media

https://doi.org/10.1006/jipa.1993.1102Get rights and content

Abstract

Two commercial serum-free media, Ex-Cell 409 and Sf900, were used to study the growth and susceptibility of Trichoplusia ni and Plutella xylostella cell lines to Autographa californica multiple nuclear polyhedrusis virus. In addition occlusion bodies produced in cell cultures were quantitated and tested in T. ni larvae for their virulence. T. ni and P. xyloslella cell lines were also grown in TC199-MK containing 10% fetal bovine serum and served as controls for comparative purposes. The doubling times for T. ni in Ex-Cell 400, Sf-900, and TC199-MK were 37, 45, and 21 hr, respectively, with a maximum cell density of 9 × 105 cells/ml in TC 199-M K. Of the three media tested, Ex-Cell 400 supported the highest viral titer (2.4 × 106 PFU/ml and produced the most occlusion bodies (17.2 × 106 OB/ml). For the P. xylostella cell line, the doubling times in Ex-Cell 400, Sf-900, and TC199-MK were 20, 27, and 32 hr, respectively, with a maximum cell density of 1.8 × 106 cells/ml in Sf-900. The highest viral titer of 12.0 × 106 PFU/ml was obtained from cells grown in Ex-Cell 400 and TC199-MK. The highest concentration of occlusion bodies (10.2 × 106/ml) was produced in cells grown in Sf-900 and TC199-MK. Occlusion bodies produced in P. xylostella cells grown in the two serum-free media and TC199-MK were equally infectious to T. ni larvae, whereas occlusion bodies produced in T. ni cells grown in Ex-Cell 400 and Sf-900 were more virulent than those grown in TC199-MK.

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