Elsevier

Experimental Cell Research

Volume 269, Issue 2, 1 October 2001, Pages 237-244
Experimental Cell Research

Regular Article
VE-Cadherin-Derived Cell-Penetrating Peptide, pVEC, with Carrier Functions

https://doi.org/10.1006/excr.2001.5316Get rights and content

Abstract

Cell-penetrating peptides, CPPs, have been shown to translocate into living cells by a receptor-independent mechanism and to carry macromolecules over the plasma membrane. This article reports studies of the internalization of pVEC, an 18-amino acid-long peptide derived from the murine sequence of the cell adhesion molecule vascular endothelial cadherin, amino acids 615–632. Fluorophore-labeled pVEC entered four different cell lines tested: human aortic endothelial cells, brain capillary endothelial cells, Bowes melanoma cells, and murine brain endothelial cells. In order to evaluate the translocation efficiency of pVEC, we performed a side-by-side comparison with penetratin, a well-characterized CPP. The cellular uptake of pVEC was highest for murine brain endothelial cells. All cell lines tested contained equal or slightly higher concentrations of pVEC than penetratin. pVEC mainly accumulated in nuclear structures but was also found throughout the cells. Furthermore, pVEC functioned as a transporter of both a hexameric peptide nucleic acid molecule of 1.7 kDa and a 67-kDa protein, streptavidin–FITC, and cellular uptake of fluorophore-labeled pVEC took place at 4°C, suggesting a nonendocytotic mechanism of translocation. In conclusion, our results indicate that pVEC is efficiently and rapidly taken up into cells and functions as a potent carrier peptide.

References (25)

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To whom correspondence and reprint requests should be addressed at Department of Neurochemistry and Neurotoxicology, Stockholm University, S-106 91, Stockholm, Sweden. Fax: + 46-8-161371. E-mail: [email protected].

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