Regular ArticleAdhesion of Lymphoid Cells to CD44-Specific Substrata: The Consequences of Attachment Depend on the Ligand
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2015, Journal of Biological ChemistryCitation Excerpt :To explore whether IL-1β induced similar changes in keratinocytes, we performed a scratch wound assay. However, IL-1β did not significantly influence the migratory activity of the keratinocytes (Fig. 6A), suggesting that a robust migration would require additional changes, perhaps enhanced synthesis of hyaluronan (49, 50) or its fixation to substratum (48, 51, 52). Because the hyaluronan coat on keratinocytes was enlarged by IL-1β, we next checked whether this influenced hyaluronan-dependent monocyte binding to the cells.
CD44 interacts directly with Lck in a zinc-dependent manner
2010, Molecular ImmunologyRole of the extracellular and cytoplasmic domains of CD44 in the rolling interaction of lymphoid cells with hyaluronan under physiologic flow
2003, Journal of Biological ChemistryCitation Excerpt :Both preparations contain high molecular-weight HA (∼1 × 103 and 4 × 103 kDa for umbilical cord HA and Healon®, respectively). Rat monoclonal antibodies (mAb) specific for mouse CD44 IM7.8.1 (referred to as IM7) and IRAWB14 (28-30) were purified from ascites fluid on protein G-Sepharose (Amersham Biosciences, Piscataway, NJ) as described (31, 32). Conjugation of antibodies with biotin (Pierce, Rockford, IL) or Alexa Fluor 594 (Molecular Probes, Eugene, OR) was performed according to the manufacturers' instructions.
Hyaluronan binding properties of a CD44 chimera containing the link module of TSG-6
2002, Journal of Biological ChemistryCitation Excerpt :The AKR1 cell line is a CD44-negative T lymphoma that binds HA constitutively when transfected with wild-type CD44 (59). CTLL-2 is a CD44-negative cytotoxic T cell line that also binds HA constitutively when transfected with CD44 (39). XJ(3)/CD44− is a CD44-negative variant of a pre-B cell line that, when transfected with wild-type CD44, shows an “inducible” HA binding phenotype (37).
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