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Light and Electron Microscope Immunocytochemical Analyses of Histone H1° Distribution in the Nucleus of Friend Erythroleukemia Cells

https://doi.org/10.1006/excr.1993.1069Get rights and content

Abstract

The localization of histone H1° in murine erythroleukemia cells which were induced to resume a differentiation program was studied in cells which have recovered their proliferative capacity after transient blockage in the G1 phase of the cell cycle. Previous studies have shown that histone H1° accumulation occurs at early times of induction and is probably related to the commitment itself. The distribution of the protein was determined by immunomicroscopy with monoclonal anti-bodies specific for histone H1°. Our observations showed that the histone accumulates in nuclei. Immunoelectron microscopy further demonstrated the presence of histone H1° in condensed chromatin areas, including perinucleolar chromatin. Moreover, histone H1° also occurred in the perichromatin regions, previously described as preferential sites of pre-mRNA synthesis, suggesting that histone H1° is not fully excluded from active chromatin.

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