Regular ArticleLight and Electron Microscope Immunocytochemical Analyses of Histone H1° Distribution in the Nucleus of Friend Erythroleukemia Cells
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Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions
2015, Journal of Biological ChemistryCitation Excerpt :In contrast, H1X is overrepresented in the coding region of non-coding RNAs, such as 45 S rRNA, miRNA, and small nucleolar RNA, possibly related to the association of this variant with transcribed genes (see below). Because previous studies have shown enrichment of H1X and H1.0 in the nucleolus of cells by immunostaining (8, 9, 54), we further explored the localization of H1 variants at nucleoli by cellular fractionation and immunoblotting. Total, cytoplasmic, nucleoplasmic, and nucleolar extracts were prepared from T47D cells.
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1996, International Review of Cytology
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