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doi:10.1006/dbio.1999.9417 
Copyright © 1999 Academic Press. All rights reserved.
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Ubiquitous Expression of Marker Transgenes in Mice and Rats
William C. Kisseberth, Nicholas T. Brettingen, Jan K. Lohse and Eric P. Sandgren1
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin–Madison, Madison, Wisconsin, 53706
Received 14 April 1999;
Abstract
The ability to unambiguously mark a cell's genotype is essential for studies in which genetically distinct cell populations must be distinguished from one another in vivo. One approach to this challenge has been the creation of transgenic mice expressing a transgene marker that is easily detectable, with no background staining. Multiple transgenic mouse strains bearing constructs with different combinations of promoter elements and coding sequences have been described, each with its own advantages and limitations. In this report we describe the use of an 800-bp promoter fragment isolated from the βgeo integration site in ROSA26 mice to target expression of two marker genes. We demonstrate that the ROSA26 promoter directs ubiquitous expression of human placental alkaline phosphatase and enhanced green fluorescent protein during embryonic and postnatal development in mouse and rat. We further demonstrate the general utility of these transgenes for marking donor cells in transplantation studies.
Author Keywords: chimera; green fluorescent protein (GFP); placental alkaline phosphatase; ROSA26; transgenic mouse; transgenic rat
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1 To whom correspondence should be addressed at Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin–Madison, 2015 Linden Drive West, Madison, WI 53706. Fax: (608) 265-8435. E-mail: sandgren@svm.vetmed.wisc.edu.
