Full PapersXLPOU-60, a Xenopus POU-Domain mRNA, Is Oocyte-Specific from Very Early Stages of Oogenesis, and Localised to Presumptive Mesoderm and Ectoderm in the Blastula
References (0)
Cited by (45)
The Xenopus Maternal-to-Zygotic Transition from the Perspective of the Germline
2015, Current Topics in Developmental BiologyCitation Excerpt :The cleavage stages before the MBT accomplish the important task of generating enough cells to begin the process of regional differentiation according to germ layer identity. Not surprisingly, these early-stage blastomeres are maintained in a pluripotent state by the expression of the maternal factors, Oct 60 and Oct 25, orthologs of mammalian Oct 3/4 (Cao et al., 2004; Cao, Siegel, & Knochel, 2006; Cao, Siegel, Oswald, & Knochel, 2008; Hinkley, Martin, Leibham, & Perry, 1992; Whitfield, Heasman, & Wylie, 1993). Post-MBT, these factors gradually decline and are replaced by lineage-specific transcription factors such as Xsox17, Bix4, GATAs, and Xnrs, many of which are activated by the maternal transcription factor, VegT, at the MBT (reviewed in Heasman, 2006; White & Heasman, 2008).
Reversal of Xenopus Oct25 function by disruption of the POU domain structure
2010, Journal of Biological ChemistryCitation Excerpt :To guarantee the correct formation of germ layers and body plan, these signals are finely tuned so that they can function at the right level and time and in the right locations. In Xenopus laevis, three Oct4 homologous factors, Oct60, Oct25, and Oct91, are expressed during oogenesis and early embryogenesis (10–12). We have found that Xenopus Oct factors play an important role in the regulation of the activities of VegT, β-catenin, and nodal and BMP4 signaling pathways and prevent premature and incorrect differentiation of embryonic cells to ensure correct formation of germ layers and of body axes (13–16).
Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation
2006, Mechanisms of DevelopmentA consensus Oct1 binding site is required for the activity of the Xenopus Cdx4 promoter
2005, Developmental BiologyNuclear factor 1 and octamer transcription factor 1 binding preset the chromatin structure of the mouse mammary tumor virus promoter for hormone induction
2004, Journal of Biological ChemistryCitation Excerpt :This suggests that a low level of endogenous Oct-binding activity is present in the oocytes. This may be in agreement with previous reports that described octamer-binding POU-domain proteins in Xenopus oocytes (41, 42). Importantly, our results show that these putative endogenous Oct-binding proteins are present in only trace amounts relative to injected DNA templates (Fig. 5, A and B) and that these endogenous proteins do not cooperate significantly with NF1 in eliciting a transcriptional response (Fig. 2) (18) or in chromatin presetting (Fig. 3).