Regular Article
Expression and Induction by IL-6 of the Normal and Variant Genes for Human Plasminogen

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Abstract

We examined the promoter activity of the gene for human plasminogen (PLG) employing its 1.1 kb fragment of the 5′-flanking region inserted in front of a reporter gene. Deletion analysis revealed that a region surrounding the transcription start site was essential for the PLG expression. Since the PLG gene has three sequences for the interleukin-6 (IL-6) responsive element, we examined the effect of IL-6 on the PLG expression. IL-6 stimulation of PLG resulted in a 2.5-fold increase in its transcription. This is also true for the PLG gene of a case with dysplasminogenemia. Although the patient's gene had six mutations in the 5′-flanking region, its promoter activity was 1.8-fold that of normal PLG.

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    Address correspondence to Akitada Ichinose, M.D., Ph.D., at Yamagata University. Fax: 81-236-28-5280.

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