Internalization ofStaphylococcus aureusby cultured osteoblasts

doi:10.1006/mpat.1995.0075

Microbial Pathogenesis

Volume 19, Issue 6, December 1995, Pages 409-419

https://doi.org/10.1006/mpat.1995.0075 Get rights and content

Abstract

Staphylococcus aureusis the most common cause of both acute and chronic osteomyelitis; however, the pathogenesis of osteomyelitis is poorly understood. We investigated the ability ofS. aureusto associate with chick osteoblasts in culture and have demonstrated internalization of bacteria by the osteoblasts. Two strains ofS. aureuswere examined that were ingested by osteoblasts to different extents, suggesting strain differences in uptake. Initial association ofS. aureusstrains with osteoblasts was independent of the presence of matrix collagen produced by the osteoblasts. Internalization of bacteria required live osteoblasts, but not liveS. aureus, indicating osteoblasts are active in ingesting the organisms. The bacteria were not killed by the osteoblasts, since viable bacteria were cultured several hours after ingestion.

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Citation Excerpt :
Mohamed et al., too, showed that the EDCC5055 and Rosenbach 1884 strains of Staphylococcus aureus were able to internalize in culture [55]. Hudson et al. [56], Jevon et al. [57], and Bosse et al. [41] also recapitulated this occurrence in vitro. The capacity for osteoblasts to become hosts for Staphylococcus aureus has also been revealed in multiple in vivo studies.
Bone and joint infections are devastating afflictions. Although medical interventions and advents have improved their care, bone and joint infections still portend dismal outcomes. Indeed, bone and joint infections are associated with extremely high mortality and morbidity rates and, generally, occur secondary to the aggressive pathogen Staphylococcus aureus. The consequences of bone and joint infections are further compounded by the fact that although they are aggressively treated, they frequently recur and result in massive bone and articular cartilage loss. Here, we review the literature and chronicle the fact that the fundamental cellular components of the musculoskeletal system can be internally infected with Staphylococcus aureus, which explains the ready recurrence of bone and joint infections even after extensive administration of antibiotic therapy and debridement and offer potential treatment solutions for further study. Moreover, we review the ramifications of intracellular infection and expound that the massive bone and articular cartilage loss is caused by the sustained proinflammatory state induced by infection and offer potential combination therapies for further study to protect bone and cartilage.
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Regular PaperInternalization ofStaphylococcus aureusby cultured osteoblasts

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Internalization ofStaphylococcus aureusby cultured osteoblasts