Regular ArticleAntiperoxidase Antibodies Enhance Refolding of Horseradish Peroxidase☆
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2011, Analytica Chimica ActaCitation Excerpt :Quantitative parameters of the A2HRP method were developed here. We first studied whether HRP activity was modified by formation of the antibody–antigen complex since both HRP folding and activity can be influenced by the interaction of HRP with the antibody [32]. HRP response was measured both for HRP alone and for various HRP–antibody ratios varying from 10:1 to 1:1000 (Fig. 4).
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Abbreviations used: AARF, antibody-assisted refolding; ABTS, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid); ELISA, enzyme-linked immunosorbent assay; HRP, horseradish peroxidase; IgG, immunoglobulin G; mAb, monoclonal antibody; PBS, 50 mM Na-phosphate buffer; pH 7.4, containing 100 mM NaCl; PBST, PBS containing 0.05% Triton X-100; TG, thyroglobulin.
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To whom correspondence and reprint requests should be addressed at present address: Department of Biochemistry and Molecular Biology, Penn State University College of Medicine, Hershey, PA 17033.