Regular Article
Expression of Peroxisome Proliferator-Activated Receptor α (PPARα) in Primary Cultures of Human Vascular Endothelial Cells

https://doi.org/10.1006/bbrc.1998.8622Get rights and content

Abstract

The peroxisome proliferator-activated receptor (PPAR) is a member of the steroid nuclear receptor superfamily. Three types of PPARs have been described in humans: PPARα, PPARβ, and PPARγ. Here we investigated the levels of PPARα mRNA in primary cultures of human umbilical venous endothelial cells (HUVEC), human umbilical arterial endothelial cells (HUAEC), human coronary arterial endothelial cells (HCAEC), and human aortic endothelial cells (HAEC), using the reverse transcriptase-polymerase chain reaction (RT-PCR). The HUVEC, HAEC, and HCAEC, but not the HUAEC, showed relatively low expression of PPARα in comparison with liver, which was used as a positive control. Moreover, the partial sequences of the PCR-amplified products from HUVEC, HAEC, and HCAEC were similar to that of the PPARα from human liver. The expression of PPARα in cultured HAEC, which were induced by dexamethasone, was inhibited by insulin. In addition, PPARα expression was also increased by bezafibrate or eicosapentaenoic acid with the physiological concentration. These results suggest that the PPARα in endothelial cells may have the same physiological role as the expression of PPARα in the liver.

References (27)

  • I. Inoue et al.

    Biochem. Biophys. Res. Commun.

    (1997)
  • T. Gebel et al.

    FEBS Lett.

    (1992)
  • J.M. Peters et al.

    J. Biol. Chem.

    (1997)
  • M. Orita et al.

    Genomics

    (1989)
  • J.C. Rodriguez et al.

    J. Biol. Chem.

    (1994)
  • N. Vu-Dac et al.

    J. Biol. Chem.

    (1994)
  • R. Hertz et al.

    J. Biol. Chem.

    (1995)
  • Y. Okuda et al.

    Biochem. Biophys. Res. Commun.

    (1997)
  • B. Zhang et al.

    J. Biol. Chem.

    (1996)
  • I. Issemann et al.

    Nature

    (1990)
  • T. Lenberger et al.

    Ann. NY Acad. Sci.

    (1996)
  • J. Arts et al.

    Arterioscler. Thromb. Vasc. Biol.

    (1997)
  • H.H. Steineger et al.

    Eur. J. Biochem.

    (1994)
  • Cited by (200)

    • Nuclear Receptors and the Adaptive Response of the Heart

      2017, Endocrinology of the Heart in Health and Disease: Integrated, Cellular, and Molecular Endocrinology of the Heart
    • Omega-3 fatty acids in neurodegenerative diseases: Focus on mitochondria

      2013, Prostaglandins Leukotrienes and Essential Fatty Acids
    View all citing articles on Scopus
    1

    All correspondence should be addressed to: Ikuo Inoue, M.D., The Fourth Department of Internal Medicine, Saitama Medical School. 38 Morohongo, Moroyama, Iruma-gun, Saitama 350-0495, Japan. Fax: +81-492-94-9752.

    View full text