Elsevier

Analytical Biochemistry

Volume 238, Issue 2, 1 July 1996, Pages 179-183
Analytical Biochemistry

Regular Article
A Combined High-Performance Liquid Chromatographic–Microbiological Assay for Serum Folic Acid

https://doi.org/10.1006/abio.1996.0272Get rights and content

Abstract

An assay of folic acid in human serum is described involving HPLC fractionation of deproteinized serum prior toLactobacillus caseiassay. High specific activity [3H]folic acid is added as internal standard to 1 ml serum prior to deproteinization with perchlorate and an aliquot is applied to a C18reverse-phase HPLC column. The folic acid fraction was assayed byL. caseimicrotiter plate assay. Intra- and interassay variations (CV) were 5.5 and 7.5% respectively for sera (n= 10) spiked with folic acid. The percentage recovery for folic acid additions of 10 and 20 ng/liter were 105.2 and 103.7%, respectively. The lower limit of detection was 1 ng folic acid/ml serum. Folates other than folic acid and 5-methyltetrahydrofolate were not detected. The assay permitted serial measurements to be made of folates in serum following an oral dose.

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