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doi:10.1006/geno.1997.4695 
Copyright © 1997 Academic Press. All rights reserved.
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Isolation of the Mouse (MFH-1) and Human (FKHL14) Mesenchyme Fork Head-1 Genes Reveals Conservation of Their Gene and Protein Structures*1, , *2
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Naoyuki Miuraa, 1, Kiyoshi Iidaa, Hideaki Kakinumaa, Xiao-Li Yanga and Toshihiro Sugiyamaa
Received 11 October 1996;
Abstract
The very recently found evolutionarily conserved DNA-binding domain of 100 amino acids, termed the fork head domain, emerged from a sequence comparison of the rat hepatocyte transcription factor HNF-3α and the homeotic genefork headofDrosophila.We previously isolated a new member of this family, the mesenchyme fork head-1 (MFH-1) gene, which is expressed in developing mesenchyme. Here we describe the isolation of the mouse (MFH-1) and human (FKHL14) chromosomal MFH-1 genes and the determination of the gene and protein structures of MFH-1. We found that the MFH-1 gene has no introns and that the identity of the amino acid sequences of mouse and human MFH-1 proteins is 94%. We also investigated the transcriptional activity of the mouse and human MFH-1 proteins and found that both proteins act as positive transactivators.
*1 Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession Nos. Y08222, Y08223, and X74040.
*2 DOC, sodium deoxycholatedpc, days postcoitum; nt, nucleotide(s); RIPA, radioimmunoprecipitation assay; SDS, sodium dodecyl sulfate;
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