Regular Article
Identification of an Orphan Receptor Gene as a Type 1 Calcitonin Gene-Related Peptide Receptor

https://doi.org/10.1006/bbrc.1995.2847Get rights and content

Abstract

Calcitonin gene-related peptide (CGRP) is a 37 residue neuropeptide that is distantly related to adrenomedullin, We have recently reported the cloning and expression of an adrenomedullin receptor which is ∼ 30% homologous to the canine orphan receptor RDC-1. Therefore we tested the hypothesis that RDC-1 was a CGRP receptor. The RDC-1 gene was expressed in COS-7 cells and showed a dose dependant increase of cAMP in response to CGRP and adrenomedullin (EC50 values of 3 × 10−9 M and 1 × 10−7 M respectively) which was inhibited by the CGRP antagonist CGRP[8-37], There was no cAMP response to amylin or [Cys(acm)2,7]α-hCGRP. Ligand binding studies confirmed high affinity of this receptor for CGRP and CGRP[8-37] with KD values of 9.2 × 10−9 M and 13.4 × 10−9 M respectively.

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