doiSerbia

Characterization of F1 interspecific hybrids between wild Helianthus annuus L. populations and cultivated sunflower

Terzić Sreten (Naučni institut za ratarstvo i povrtarstvo, Novi Sad)
Atlagić Jovanka (Naučni institut za ratarstvo i povrtarstvo, Novi Sad)
Panković Dejana (Naučni institut za ratarstvo i povrtarstvo, Novi Sad)

Phenotype, chromosomes pairing and pollen vitality were compared between parental populations and F1 hybrids of interspecific cross between Helianthus annuus L. and cultivated sunflower. The investigation of the simple sequence repeats (SSR) polymorphism was also used to test the hybrid nature of F1 populations. The phenotypic traits of F1 hybrid plants were either closer to the wild species or intermediate. Irregular chromosome pairing was found in only 0 to 10% of meiocytes in the meiosis of F1 hybrid plants. Interspecific crosses were confirmed with SSR markers in all hybrid combinations. Alleles that were not present in parental DNA were frequently observed in F1 hybrids. That is additional evidence that those hybrid combinations were not produced by self-fertilization. The results suggest that SSR markers can be efficiently used for the F1 hybrid characterization in crosses between closely related species, in which, the changes of phenotype, meiosis and pollen vitality are not always significant.

Keywords: sunflower, wild species, interspecific hybridization, phenotype, analysis of meiosis, pollen viability, SSR