Issue 16, 2010
From the journal:

Lab on a Chip

Three-dimensional photopatterning of hydrogels using stereolithography for long-term cell encapsulation†

Vincent Chan,ad   Pinar Zorlutuna,ad   Jae Hyun Jeong,b   Hyunjoon Kongb  and  Rashid Bashir*acd  

* Corresponding authors

a Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA

b Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA

c Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA

d 2000 Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, MC-249, 208 North Wright Street, Urbana, Illinois, USA
E-mail: rbashir@illinois.edu
Fax: +1 (217) 244-6375
Tel: +1 (217) 333-3097

Abstract

Cell-encapsulated hydrogels with complex three-dimensional (3D) structures were fabricated from photopolymerizable poly(ethylene glycol) diacrylate (PEGDA) using modified ‘top-down’ and ‘bottoms-up’ versions of a commercially available stereolithography apparatus (SLA). Swelling and mechanical properties were measured for PEGDA hydrogels with molecular weights (Mw) ranging from 700 to 10 000 Daltons (Da). Long-term viability of encapsulated NIH/3T3 cells was quantitatively evaluated using an MTS assay and shown to improve over 14 days by increasing the Mw of the hydrogels. Addition of adhesive RGDS peptide sequences resulted in increased cell viability, proliferation, and spreading compared to pristine PEG hydrogels of the same Mw. Spatial 3D layer-by-layer cell patterning was successfully demonstrated, and the feasibility of depositing multiple cell types and material compositions into distinct layers was established.

Graphical abstract: Three-dimensional photopatterning of hydrogels using stereolithography for long-term cell encapsulation

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Article information

DOI
https://doi.org/10.1039/C004285D
Article type
Paper
Submitted
16 Mar 2010
Accepted
11 Jun 2010
First published
05 Jul 2010

Lab Chip, 2010,10, 2062-2070

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Three-dimensional photopatterning of hydrogels using stereolithography for long-term cell encapsulation

V. Chan, P. Zorlutuna, J. H. Jeong, H. Kong and R. Bashir, Lab Chip, 2010, 10, 2062 DOI: 10.1039/C004285D

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